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dc.contributor.author | Takimoto, Ren | en |
dc.contributor.author | Tatemichi, Yuki | en |
dc.contributor.author | Aoki, Wataru | en |
dc.contributor.author | Kosaka, Yuishin | en |
dc.contributor.author | Minakuchi, Hiroyoshi | en |
dc.contributor.author | Ueda, Mitsuyoshi | en |
dc.contributor.author | Kuroda, Kouichi | en |
dc.contributor.alternative | 滝本, 廉 | ja |
dc.contributor.alternative | 立道, 祐輝 | ja |
dc.contributor.alternative | 青木, 航 | ja |
dc.contributor.alternative | 小坂, 唯心 | ja |
dc.contributor.alternative | 水口, 博義 | ja |
dc.contributor.alternative | 植田, 充美 | ja |
dc.contributor.alternative | 黒田, 浩一 | ja |
dc.date.accessioned | 2022-03-18T01:17:15Z | - |
dc.date.available | 2022-03-18T01:17:15Z | - |
dc.date.issued | 2022 | - |
dc.identifier.uri | http://hdl.handle.net/2433/268930 | - |
dc.description | 酸素に弱いニトロゲナーゼを有酸素条件でも機能させるための重要因子を発見 --化石燃料を必要としない脱炭素社会の基盤形成に向け、空中窒素の固定を介した生物学的アンモニア生産へ期待--. 京都大学プレスリリース. 2022-03-17. | ja |
dc.description.abstract | Since nitrogenase is irreversibly inactivated within a few minutes after exposure to oxygen, current studies on the heterologous expression of nitrogenase are limited to anaerobic conditions. This study comprehensively identified genes showing oxygen-concentration-dependent expression only under nitrogen-fixing conditions in Azotobacter vinelandii, an aerobic diazotroph. Among the identified genes, nafU, with an unknown function, was greatly upregulated under aerobic nitrogen-fixing conditions. Through replacement and overexpressing experiments, we suggested that nafU is involved in the maintenance of nitrogenase activity under aerobic nitrogenase activity. Furthermore, heterologous expression of nafU in nitrogenase-producing Escherichia coli increased nitrogenase activity under aerobic conditions by 9.7 times. Further analysis of NafU protein strongly suggested its localization in the inner membrane and raised the possibility that this protein may lower the oxygen concentration inside the cells. These findings provide new insights into the mechanisms for maintaining stable nitrogenase activity under aerobic conditions in A. vinelandii and provide a platform to advance the use of nitrogenase under aerobic conditions. | en |
dc.language.iso | eng | - |
dc.publisher | Springer Nature | en |
dc.rights | © The Author(s) 2022 | en |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Microbiology | en |
dc.subject | Molecular biology | en |
dc.title | A critical role of an oxygen-responsive gene for aerobic nitrogenase activity in Azotobacter vinelandii and its application to Escherichia coli | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Scientific Reports | en |
dc.identifier.volume | 12 | - |
dc.relation.doi | 10.1038/s41598-022-08007-4 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 4182 | - |
dc.address | Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University | en |
dc.address | Research and Development Division, Kikkoman Corporation | en |
dc.address | Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University | en |
dc.address | Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University | en |
dc.address | Kyoto Monotech | en |
dc.address | Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University | en |
dc.address | Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University | en |
dc.identifier.pmid | 35264690 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2022-03-17 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | JPMJPF2008 | - |
datacite.awardNumber.uri | https://projectdb.jst.go.jp/grant/JST-PROJECT-20354791/ | - |
dc.identifier.eissn | 2045-2322 | - |
jpcoar.funderName | 科学技術振興機構 | ja |
jpcoar.awardTitle | 食サイクルのイノベーション(フード&アグリテック)未来共創拠点 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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