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dc.contributor.author | Miura, Yasutomo | en |
dc.contributor.author | Sato, Masae | en |
dc.contributor.author | Kuwahara, Toshie | en |
dc.contributor.author | Ebata, Tomoki | en |
dc.contributor.author | Tabata, Yasuhiko | en |
dc.contributor.author | Sakurai, Hidetoshi | en |
dc.contributor.alternative | 三浦, 泰智 | ja |
dc.contributor.alternative | 佐藤, 優江 | ja |
dc.contributor.alternative | 桑原, 寿江 | ja |
dc.contributor.alternative | 江畑, 智希 | ja |
dc.contributor.alternative | 田畑, 泰彦 | ja |
dc.contributor.alternative | 櫻井, 英俊 | ja |
dc.date.accessioned | 2022-04-25T05:27:34Z | - |
dc.date.available | 2022-04-25T05:27:34Z | - |
dc.date.issued | 2022-04 | - |
dc.identifier.uri | http://hdl.handle.net/2433/269452 | - |
dc.description | マウスの横隔膜にヒトiPS細胞から作った骨格筋幹細胞を移植する --デュシェンヌ型筋ジストロフィーの呼吸筋治療に向けた横隔膜移植方法の確立--. 京都大学プレスリリース. 2022-04-13. | ja |
dc.description.abstract | Duchenne muscular dystrophy (DMD) is an intractable genetic muscular disorder characterized by the loss of DYSTROPHIN. The restoration of DYSTROPHIN is expected to be a curative therapy for DMD. Because muscle stem cells (MuSCs) can regenerate damaged myofibers with full-length DYSTROPHIN in vivo, their transplantation is being explored as such a therapy. As for the transplanted cells, primary satellite cells have been considered, but donor shortage limits their clinical application. We previously developed a protocol that differentiates induced pluripotent stem cells (iPSCs) to MuSCs (iMuSCs). To ameliorate the respiratory function of DMD patients, cell transplantation to the diaphragm is necessary but difficult, because the diaphragm is thin and rapidly moves. In the present study, we explored the transplantation of iMuSCs into the diaphragm. First, we show direct cell injection into the diaphragm of mouse was feasible. Then, to enhance the engraftment of the transplanted cells in a rapidly moving diaphragm, we mixed polymer solutions of hyaluronic acid, alginate and gelatin to the cell suspension, finding a solution of 20% dissolved hyaluronic acid and 80% dissolved gelatin improved the engraftment. Thus, we established a method for cell transplantation into mouse diaphragm and show that an injectable hyaluronic acid-gelatin solution enables the engraftment of iMuSCs in the diaphragm. | en |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | en |
dc.rights | Copyright: © 2022 Miura et al. | en |
dc.rights | This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Thoracic diaphragm | en |
dc.subject | Polymers | en |
dc.subject | Cell transplantation | en |
dc.subject | Immunohistochemistry techniques | en |
dc.subject | DAPI staining | en |
dc.subject | Dystrophin | en |
dc.subject | Gelatin | en |
dc.subject | Stem cell transplantation | en |
dc.title | Transplantation of human iPSC-derived muscle stem cells in the diaphragm of Duchenne muscular dystrophy model mice | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | PLOS ONE | en |
dc.identifier.volume | 17 | - |
dc.identifier.issue | 4 | - |
dc.relation.doi | 10.1371/journal.pone.0266391 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | e0266391 | - |
dc.address | Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University; Divison of Surgical Oncology, Department of Surgery, Nagoya University Graduate School of Medicine | en |
dc.address | Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University | en |
dc.address | Laboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical Science, Kyoto University | en |
dc.address | Divison of Surgical Oncology, Department of Surgery, Nagoya University Graduate School of Medicine | en |
dc.address | Laboratory of Biomaterials, Department of Regeneration Science and Engineering, Institute for Frontier Life and Medical Science, Kyoto University | en |
dc.address | Department of Clinical Application, Center for iPS Cell Research and Application (CiRA), Kyoto University | en |
dc.identifier.pmid | 35377913 | - |
dc.relation.url | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/220413-130000.html | - |
dcterms.accessRights | open access | - |
dc.identifier.eissn | 1932-6203 | - |
出現コレクション: | 学術雑誌掲載論文等 |
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