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eLife.67659.pdf | 8.53 MB | Adobe PDF | 見る/開く |
タイトル: | High and stable ATP levels prevent aberrant intracellular protein aggregation in yeast |
著者: | Takaine, Masak Imamura, Hiromi ![]() ![]() ![]() Yoshida, Satoshi |
著者名の別形: | 高稲, 正勝 今村, 博臣 吉田, 知史 |
キーワード: | Research Article Cell Biology ATP proteostasis AMPK adenylate kinase yeast homeostasis S. cerevisiae |
発行日: | 2022 |
出版者: | eLife Sciences Publications, Ltd |
誌名: | eLife |
巻: | 11 |
論文番号: | e67659 |
抄録: | Adenosine triphosphate (ATP) at millimolar levels has recently been implicated in the solubilization of cellular proteins. However, the significance of this high ATP level under physiological conditions and the mechanisms that maintain ATP remain unclear. We herein demonstrated that AMP-activated protein kinase (AMPK) and adenylate kinase (ADK) cooperated to maintain cellular ATP levels regardless of glucose levels. Single-cell imaging of ATP-reduced yeast mutants revealed that ATP levels in these mutants underwent stochastic and transient depletion, which promoted the cytotoxic aggregation of endogenous proteins and pathogenic proteins, such as huntingtin and α-synuclein. Moreover, pharmacological elevations in ATP levels in an ATP-reduced mutant prevented the accumulation of α-synuclein aggregates and its cytotoxicity. The present study demonstrates that cellular ATP homeostasis ensures proteostasis and revealed that suppressing the high volatility of cellular ATP levels prevented cytotoxic protein aggregation, implying that AMPK and ADK are important factors that prevent proteinopathies, such as neurodegenerative diseases. |
記述: | 高濃度のATPがタンパク質の異常な凝集を防ぐ --細胞内ATPの新たな役割を発見、神経変性疾患の発症に関与する可能性--. 京都大学プレスリリース. 2022-04-25. |
著作権等: | © 2022, Takaine et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. |
URI: | http://hdl.handle.net/2433/269472 |
DOI(出版社版): | 10.7554/eLife.67659 |
PubMed ID: | 35438635 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2022-04-25 |
出現コレクション: | 学術雑誌掲載論文等 |
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