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ファイル | 記述 | サイズ | フォーマット | |
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j.crmeth.2022.100215.pdf | 1.98 MB | Adobe PDF | 見る/開く |
タイトル: | DIPA-CRISPR is a simple and accessible method for insect gene editing |
著者: | Shirai, Yu Piulachs, Maria-Dolors Belles, Xavier Daimon, Takaaki |
著者名の別形: | 白井, 雄 大門, 高明 |
キーワード: | CRISPR-Cas9 DIPA-CRISPR gene editing genome editing cockroaches beetles |
発行日: | 23-May-2022 |
出版者: | Elsevier BV |
誌名: | Cell Reports Methods |
巻: | 2 |
号: | 5 |
論文番号: | 100215 |
抄録: | Current approaches for insect gene editing require microinjection of materials into early embryos. This severely limits the application of gene editing to a great number of insect species, especially to those whose reproduction systems preclude access to early embryos for injection. To overcome these limitations, we report a simple and accessible method for insect gene editing, termed “direct parental” CRISPR (DIPA-CRISPR). We show that injection of Cas9 ribonucleoproteins (RNPs) into the haemocoel of adult females efficiently introduces heritable mutations in developing oocytes. Importantly, commercially available standard Cas9 protein can be directly used for DIPA-CRISPR, which makes this approach highly practical and feasible. DIPA-CRISPR enables highly efficient gene editing in the cockroaches, on which conventional approaches cannot be applied, and in the model beetle Tribolium castaneum. Due to its simplicity and accessibility, DIPA-CRISPR will greatly extend the application of gene editing technology to a wide variety of insects. |
記述: | 昆虫ゲノム編集のあたらしい形 --成虫注射で「難敵」撃破--. 京都大学プレスリリース. 2022-05-18. |
著作権等: | © 2022 The Author(s). This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. |
URI: | http://hdl.handle.net/2433/273977 |
DOI(出版社版): | 10.1016/j.crmeth.2022.100215 |
PubMed ID: | 35637909 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2022-05-18-0 |
出現コレクション: | 学術雑誌掲載論文等 |
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