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dc.contributor.authorShinoda, Hajimeen
dc.contributor.authorIida, Tatsuyaen
dc.contributor.authorMakino, Asamien
dc.contributor.authorYoshimura, Mamien
dc.contributor.authorIshikawa, Junichiroen
dc.contributor.authorAndo, Junen
dc.contributor.authorMurai, Kazueen
dc.contributor.authorSugiyama, Katsumien
dc.contributor.authorMuramoto, Yukikoen
dc.contributor.authorNakano, Masahiroen
dc.contributor.authorKiga, Kotaroen
dc.contributor.authorCui, Longzhuen
dc.contributor.authorNureki, Osamuen
dc.contributor.authorTakeuchi, Hiroakien
dc.contributor.authorNoda, Takeshien
dc.contributor.authorNishimasu, Hiroshien
dc.contributor.authorWatanabe, Rikiyaen
dc.contributor.alternative篠田, 肇ja
dc.contributor.alternative飯田, 龍也ja
dc.contributor.alternative牧野, 麻美ja
dc.contributor.alternative吉村, 麻実ja
dc.contributor.alternative石川, 潤一郎ja
dc.contributor.alternative安藤, 潤ja
dc.contributor.alternative村井, 和枝ja
dc.contributor.alternative杉山, 勝美ja
dc.contributor.alternative村本, 裕紀子ja
dc.contributor.alternative中野, 雅博ja
dc.contributor.alternative氣駕, 恒太朗ja
dc.contributor.alternative崔, 龍洙ja
dc.contributor.alternative濡木, 理ja
dc.contributor.alternative武内, 寛明ja
dc.contributor.alternative野田, 岳志ja
dc.contributor.alternative西増, 弘志ja
dc.contributor.alternative渡邉, 力也ja
dc.date.accessioned2022-05-29T23:57:26Z-
dc.date.available2022-05-29T23:57:26Z-
dc.date.issued2022-
dc.identifier.urihttp://hdl.handle.net/2433/274105-
dc.description新型コロナウイルスの超高感度・全自動迅速検出装置の開発 --汎用的な感染症診断装置としての社会実装に期待--. 京都大学プレスリリース. 2022-05-27.ja
dc.description.abstractIn the ongoing COVID-19 pandemic, rapid and sensitive diagnosis of viral infection is a critical deterrent to the spread of SARS-CoV-2. To this end, we developed an automated amplification-free digital RNA detection platform using CRISPR-Cas13a and microchamber device (opn-SATORI), which automatically completes a detection process from sample mixing to RNA quantification in clinical specimens within ~9 min. Using the optimal Cas13a enzyme and magnetic beads technology, opn-SATORI detected SARS-CoV-2 genomic RNA with a LoD of < 6.5 aM (3.9 copies μL⁻¹), comparable to RT-qPCR. Additionally, opn-SATORI discriminated between SARS-CoV-2 variants of concern, including alpha, delta, and omicron, with 98% accuracy. Thus, opn-SATORI can serve as a rapid and convenient diagnostic platform for identifying several types of viral infections.en
dc.language.isoeng-
dc.publisherSpringer Natureen
dc.rights© The Author(s) 2022en
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/-
dc.subjectLab-on-a-chipen
dc.subjectNanobiotechnologyen
dc.subjectSingle-molecule biophysicsen
dc.titleAutomated amplification-free digital RNA detection platform for rapid and sensitive SARS-CoV-2 diagnosisen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleCommunications Biologyen
dc.identifier.volume5-
dc.relation.doi10.1038/s42003-022-03433-6-
dc.textversionpublisher-
dc.identifier.artnum473-
dc.addressMolecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN; CREST, Japan Science and Technology Agencyen
dc.addressMolecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN; CREST, Japan Science and Technology Agencyen
dc.addressMolecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN; CREST, Japan Science and Technology Agencyen
dc.addressMolecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN; CREST, Japan Science and Technology Agencyen
dc.addressDepartment of Biological Sciences, Graduate School of Science, The University of Tokyoen
dc.addressMolecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN; CREST, Japan Science and Technology Agencyen
dc.addressMolecular Physiology Laboratory, Cluster for Pioneering Research, RIKENen
dc.addressFUJIFILM Media Crest Co., Ltden
dc.addressCREST, Japan Science and Technology Agency; Institute for Frontier Life and Medical Sciences, Kyoto Universityen
dc.addressCREST, Japan Science and Technology Agency; Institute for Frontier Life and Medical Sciences, Kyoto Universityen
dc.addressDivision of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical University; Research Center for Drug and Vaccine Development, National Institute of Infectious Diseasesen
dc.addressDivision of Bacteriology, Department of Infection and Immunity, School of Medicine, Jichi Medical Universityen
dc.addressDepartment of Biological Sciences, Graduate School of Science, The University of Tokyoen
dc.addressDepartment of Molecular Virology, Tokyo Medical and Dental Universityen
dc.addressCREST, Japan Science and Technology Agency; Institute for Frontier Life and Medical Sciences, Kyoto Universityen
dc.addressCREST, Japan Science and Technology Agency; Department of Biological Sciences, Graduate School of Science, The University of Tokyo; Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo; Research Center for Advanced Science and Technology, Structural Biology Division, The University of Tokyo; Inamori Research Institute for Scienceen
dc.addressMolecular Physiology Laboratory, Cluster for Pioneering Research, RIKEN; CREST, Japan Science and Technology Agencyen
dc.identifier.pmid35614128-
dc.relation.urlhttps://www.kyoto-u.ac.jp/ja/research-news/2022-05-27-2-
dcterms.accessRightsopen access-
datacite.awardNumber21H04645-
datacite.awardNumber20H05931-
datacite.awardNumber.urihttps://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H04645/-
datacite.awardNumber.urihttps://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-20H05931/-
dc.identifier.eissn2399-3642-
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.awardTitleデジタル検出技術を基盤としたウイルスの新規感染診断法の開発ja
jpcoar.awardTitle未開拓タンパク質の1分子計測技術・デバイス開発ja
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