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|---|---|---|---|---|
| cbic.202100533.pdf | 763.96 kB | Adobe PDF | View/Open |
| Title: | Strong and Specific Recognition of CAG/CTG Repeat DNA (5'-dWGCWGCW-3') by a Cyclic Pyrrole-Imidazole Polyamide |
| Authors: | Hirose, Yuki Ohno, Tomo Asamitsu, Sefan Hashiya, Kaori Bando, Toshikazu   Sugiyama, Hiroshi  https://orcid.org/0000-0001-8923-5946 (unconfirmed) |
| Author's alias: | 廣瀬, 優希 大野, 智 朝光, 世煌 橋谷, かおり 板東, 俊和 杉山, 弘 |
| Keywords: | CAG/CTG repeats DNA recognition pyrrole-imidazole polyamides triplet repeats |
| Issue Date: | 19-Jan-2022 |
| Publisher: | Wiley |
| Journal title: | ChemBioChem |
| Volume: | 23 |
| Issue: | 2 |
| Thesis number: | e202100533 |
| Abstract: | Abnormally expanded CAG/CTG repeat DNA sequences lead to a variety of neurological diseases, such as Huntington's disease. Here, we synthesized a cyclic pyrrole-imidazole polyamide (cPIP), which can bind to the minor groove of the CAG/CTG DNA sequence. The double-stranded DNA melting temperature (Tm ) and surface plasmon resonance assays revealed the high binding affinity of the cPIP. In addition, next-generation sequencing showed that the cPIP had high specificity for its target DNA sequence. |
| Rights: | © 2021 The Authors. ChemBioChem published by Wiley-VCH GmbH This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
| URI: | http://hdl.handle.net/2433/276072 |
| DOI(Published Version): | 10.1002/cbic.202100533 |
| PubMed ID: | 34796607 |
| Appears in Collections: | Journal Articles |
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