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j.virol.2022.01.015.pdf | 9.86 MB | Adobe PDF | 見る/開く |
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DCフィールド | 値 | 言語 |
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dc.contributor.author | Takata, Shota | en |
dc.contributor.author | Mise, Kazuyuki | en |
dc.contributor.author | Takano, Yoshitaka | en |
dc.contributor.author | Kaido, Masanori | en |
dc.contributor.alternative | 高田, 昌汰 | ja |
dc.contributor.alternative | 三瀬, 和之 | ja |
dc.contributor.alternative | 髙野, 義孝 | ja |
dc.contributor.alternative | 海道, 真典 | ja |
dc.date.accessioned | 2022-09-08T04:40:21Z | - |
dc.date.available | 2022-09-08T04:40:21Z | - |
dc.date.issued | 2022-03 | - |
dc.identifier.uri | http://hdl.handle.net/2433/276202 | - |
dc.description.abstract | New evidences are emerging to support the importance of viral replication complexes (VRCs) in not only viral replication, but also viral cell-to-cell movement. Currently, how VRCs grow in size and colocalize with viral movement proteins (MPs) remains unclear. Herein, we performed live-cell imaging of red clover necrotic mosaic virus (RCNMV) dsRNA by using reporter B2-GFP plants. Tiny granules of dsRNA were formed along the endoplasmic reticulum (ER) at an early stage of infection. Importantly, the colocalization of the dsRNA granules with the virus-encoded p27 replication protein showed that these structures are components of VRCs. These granules moved throughout the cytoplasm, driven by the acto–myosin system, and coalesced with each other to form larger aggregates; the MPs were not associated with these processes. Notably, the MPs colocalized preferentially with large dsRNA aggregates, rather than with tiny dsRNA granules, suggesting that the increase in the size of VRCs promotes their colocalization with MPs. | en |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2022 The Authors. Published by Elsevier Inc. | en |
dc.rights | This is an open access article under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International license. | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | Viral replication complex (VRC) | en |
dc.subject | Movement protein | en |
dc.subject | Positive-strand RNA virus | en |
dc.subject | Dianthovirus | en |
dc.subject | Endoplasmic reticulum | en |
dc.title | Subcellular dynamics of red clover necrotic mosaic virus double-stranded RNAs in infected plant cells | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Virology | en |
dc.identifier.volume | 568 | - |
dc.identifier.spage | 126 | - |
dc.identifier.epage | 139 | - |
dc.relation.doi | 10.1016/j.virol.2022.01.015 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 35180583 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 16H04882 | - |
datacite.awardNumber | 21H02199 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16H04882/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H02199/ | - |
dc.identifier.pissn | 0042-6822 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 植物RNAウイルスの輸送ハブ形成過程と細胞間移行機構の解明 | ja |
jpcoar.awardTitle | 植物RNAウイルス移行タンパク質の構造解明と輸送ハブの形成機構 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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