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sciadv.abc2648.pdf | 5.03 MB | Adobe PDF | 見る/開く |
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DCフィールド | 値 | 言語 |
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dc.contributor.author | Hippler, Marc | en |
dc.contributor.author | Weißenbruch, Kai | en |
dc.contributor.author | Richler, Kai | en |
dc.contributor.author | Lemma, Enrico D. | en |
dc.contributor.author | Nakahata, Masaki | en |
dc.contributor.author | Richter, Benjamin | en |
dc.contributor.author | Barner-Kowollik, Christopher | en |
dc.contributor.author | Takashima, Yoshinori | en |
dc.contributor.author | Harada, Akira | en |
dc.contributor.author | Blasco, Eva | en |
dc.contributor.author | Wegener, Martin | en |
dc.contributor.author | Tanaka, Motomu | en |
dc.contributor.author | Bastmeyer, Martin | en |
dc.contributor.alternative | 田中, 求 | ja |
dc.date.accessioned | 2022-09-16T07:59:25Z | - |
dc.date.available | 2022-09-16T07:59:25Z | - |
dc.date.issued | 2020-09 | - |
dc.identifier.uri | http://hdl.handle.net/2433/276315 | - |
dc.description.abstract | Many essential cellular processes are regulated by mechanical properties of their microenvironment. Here, we introduce stimuli-responsive composite scaffolds fabricated by three-dimensional (3D) laser lithography to simultaneously stretch large numbers of single cells in tailored 3D microenvironments. The key material is a stimuli-responsive photoresist containing cross-links formed by noncovalent, directional interactions between β-cyclodextrin (host) and adamantane (guest). This allows reversible actuation under physiological conditions by application of soluble competitive guests. Cells adhering in these scaffolds build up initial traction forces of ~80 nN. After application of an equibiaxial stretch of up to 25%, cells remodel their actin cytoskeleton, double their traction forces, and equilibrate at a new dynamic set point within 30 min. When the stretch is released, traction forces gradually decrease until the initial set point is retrieved. Pharmacological inhibition or knockout of nonmuscle myosin 2A prevents these adjustments, suggesting that cellular tensional homeostasis strongly depends on functional myosin motors. | en |
dc.language.iso | eng | - |
dc.publisher | American Association for the Advancement of Science (AAAS) | en |
dc.rights | © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). | en |
dc.rights | This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license, which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. | en |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/ | - |
dc.title | Mechanical stimulation of single cells by reversible host-guest interactions in 3D microscaffolds | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Science Advances | en |
dc.identifier.volume | 6 | - |
dc.identifier.issue | 39 | - |
dc.relation.doi | 10.1126/sciadv.abc2648 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | eabc2648 | - |
dc.identifier.pmid | 32967835 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 17H00855 | - |
datacite.awardNumber | 19H05719 | - |
datacite.awardNumber | 20H00661 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-17H00855/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PLANNED-19H05719/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-20H00661/ | - |
dc.identifier.eissn | 2375-2548 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 微小環境の動的変化を用いた幹細胞の機能制御技術 | ja |
jpcoar.awardTitle | 水圏機能材料の電子・イオン機能開拓 | ja |
jpcoar.awardTitle | がん細胞・オルガノイドの動態を基盤とする物理的バイオマーカーの創出 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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