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dc.contributor.authorOmae, Kimihoen
dc.contributor.authorOguro, Tatsukien
dc.contributor.authorInoue, Masaoen
dc.contributor.authorFukuyama, Yutoen
dc.contributor.authorYoshida, Takashien
dc.contributor.authorSako, Yoshihikoen
dc.contributor.alternative大前, 公保ja
dc.contributor.alternative大黒, 達希ja
dc.contributor.alternative井上, 真男ja
dc.contributor.alternative福山, 宥斗ja
dc.contributor.alternative吉田, 天士ja
dc.contributor.alternative左子, 芳彦ja
dc.date.accessioned2022-10-03T07:01:56Z-
dc.date.available2022-10-03T07:01:56Z-
dc.date.issued2021-01-
dc.identifier.urihttp://hdl.handle.net/2433/276520-
dc.description.abstractThe microbial H2-producing (hydrogenogenic) carbon monoxide (CO)-oxidizing activity by the membrane-associated CO dehydrogenase (CODH)/energy-converting hydrogenase (ECH) complex is an important metabolic process in the microbial community. However, the studies on hydrogenogenic carboxydotrophs had to rely on inherently cultivation and isolation methods due to their rare abundance, which was a bottleneck in ecological study. Here, we provided gene-targeted sequencing method for the diversity estimation of thermophilic hydrogenogenic carboxydotrophs. We designed six new degenerate primer pairs which effectively amplified the coding regions of CODH genes forming gene clusters with ECH genes (CODHech genes) in Firmicutes which includes major thermophilic hydrogenogenic carboxydotrophs in terrestrial thermal habitats. Amplicon sequencing by these primers using DNAs from terrestrial hydrothermal sediments and CO-gas-incubated samples specifically detected multiple CODH genes which were identical or phylogenetically related to the CODHech genes in Firmictes. Furthermore, we found that phylogenetically distinct CODHech genes were enriched in CO-gas-incubated samples, suggesting that our primers detected uncultured hydrogenogenic carboxydotrophs as well. The new CODH-targeted primers provided us with a fine-grained (~ 97.9% in nucleotide sequence identity) diversity analysis of thermophilic hydrogenogenic carboxydotrophs by amplicon sequencing and will bolster the ecological study of these microorganisms.en
dc.language.isoeng-
dc.publisherSpringer Natureen
dc.rights© The Author(s) 2021en
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.en
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/-
dc.subjectCarbon monoxide dehydrogenaseen
dc.subjectCarboxydotrophen
dc.subjectHydrogen productionen
dc.subjectGroup 4 [NiFe] hydrogenaseen
dc.subjectThermophileen
dc.subjectAmplicon sequencingen
dc.subjectRare biosphereen
dc.titleDiversity analysis of thermophilic hydrogenogenic carboxydotrophs by carbon monoxide dehydrogenase amplicon sequencing using new primersen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleExtremophilesen
dc.identifier.volume25-
dc.identifier.issue1-
dc.identifier.spage61-
dc.identifier.epage76-
dc.relation.doi10.1007/s00792-020-01211-y-
dc.textversionpublisher-
dc.identifier.pmid33415441-
dcterms.accessRightsopen access-
datacite.awardNumber25252038-
datacite.awardNumber16H06381-
datacite.awardNumber16J11269-
datacite.awardNumber.urihttps://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25252038/-
datacite.awardNumber.urihttps://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16H06381/-
datacite.awardNumber.urihttps://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16J11269/-
dc.identifier.pissn1431-0651-
dc.identifier.eissn1433-4909-
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.awardTitle一酸化炭素の次世代活用に関する分子微生物学的研究ja
jpcoar.awardTitle時空間的探索による一酸化炭素資化菌の包括的研究とその応用基盤の構築ja
jpcoar.awardTitle時空間的探索による一酸化炭素資化菌の包括的研究とその応用基盤の構築ja
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