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タイトル: | Synthetic Mitochondria-Targeting Peptides Incorporating α-Aminoisobutyric Acid with a Stable Amphiphilic Helix Conformation in Plant Cells |
著者: | Terada, Kayo Gimenez-Dejoz, Joan Kurita, Taichi Oikawa, Kazusato Uji, Hirotaka ![]() ![]() Tsuchiya, Kousuke Numata, Keiji ![]() ![]() ![]() |
著者名の別形: | 寺田, 佳世 栗田, 太一 及川, 和聡 宇治, 広隆 土屋, 康佑 沼田, 圭司 |
キーワード: | α-aminoisobutyric acid amphiphilic helix synthetic mitochondria-targeting peptide Tom20-recognition consensus sequence |
発行日: | 12-Apr-2021 |
出版者: | American Chemical Society (ACS) |
誌名: | ACS Biomaterials Science & Engineering |
巻: | 7 |
号: | 4 |
開始ページ: | 1475 |
終了ページ: | 1484 |
抄録: | In the genetic modification of plant cells, the mitochondrion is an important target in addition to the nucleus and plastid. However, gene delivery into the mitochondria of plant cells has yet to be established by conventional methods, such as particle bombardment, because of the small size and high mobility of mitochondria. To develop an efficient mitochondria-targeting signal (MTS) that functions in plant cells, we designed the artificial peptide (LURL)₃ and its analogues, which periodically feature hydrophobic α-aminoisobutyric acid (Aib, U) and cationic arginine (R), considering the consensus motif recognized by the mitochondrial import receptor Tom20. Circular dichroism measurements and molecular dynamics simulation studies revealed that (LURL)₃ had a propensity to form a stable α-helix in 0.1 M phosphate buffer solution containing 1.0 wt % sodium dodecyl sulfate. After internalization into plant cells via particle bombardment, (LURL)₃ revealed highly selective accumulation in the mitochondria, whereas its analogue (LARL)₃ was predominantly located in the vacuoles in addition to mitochondria. The high selectivity of (LURL)₃ can be attributed to the incorporation of Aib, which promotes the hydrophobic interaction between the MTS and Tom20 by increasing the hydrophobicity and helicity of (LURL)₃. The present study provided a prospective mitochondrial targeting system using the simple design of artificial peptides. |
著作権等: | Copyright © 2021 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial NoDerivative Works (CC-BY-NC-ND) Attribution License, which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
URI: | http://hdl.handle.net/2433/276532 |
DOI(出版社版): | 10.1021/acsbiomaterials.0c01533 |
PubMed ID: | 33606492 |
出現コレクション: | 学術雑誌掲載論文等 |
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