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j.bbrc.2022.04.103.pdf | 2 MB | Adobe PDF | 見る/開く |
タイトル: | Enhancement of vitamin C-induced myogenesis by inhibition of extracellular signal-regulated kinase (ERK) 1/2 pathway |
著者: | Fu, Xiajie Matsui, Tohru Funaba, Masayuki ![]() ![]() ![]() |
著者名の別形: | 松井, 徹 舟場, 正幸 |
キーワード: | Myogenesis Vitamin C ERK1/2 p38 |
発行日: | 5-Jul-2022 |
出版者: | Elsevier BV |
誌名: | Biochemical and Biophysical Research Communications |
巻: | 612 |
開始ページ: | 57 |
終了ページ: | 62 |
抄録: | Myogenesis is a complex process that is regulated by a variety of factors. We have previously shown that vitamin C and mild endoplasmic reticulum stress synergistically enhance myogenesis. The present study evaluated the effects of vitamin C (ascorbic acid (AsA) and AsA 2-phosphate (AsAp)) and extracellular signal-regulated kinase (ERK) 1/2 pathway on myogenesis. Treatment with U0126, an inhibitor of MEK1/2 that phosphorylates and activates ERK1/2, during the differentiation, increased the mRNA levels of Myod and Myog with an increase in the protein level of myosin heavy chain (MYH)1/2. Treatment with AsA or AsAp alone had minimal effects on myogenesis in C2C12 cells. However, combination treatment with vitamin C and U0126 greatly enhanced myogenesis; the number of thick and long myotubes was increased, and the expression of MYH1/2 was also increased. PD98059, another MEK1/2 inhibitor, also enhanced myogenesis in combination with vitamin C. These results indicate that relief of endogenous ERK1/2 activity enhances vitamin C-mediated myogenesis, suggesting a functional interaction between endogenous ERK1/2 activity and vitamin C. In addition, inhibition of p38 mitogen-activated protein kinase repressed myogenesis in the presence of vitamin C. Thus, vitamin C is a conditional factor that modulates myogenesis. |
著作権等: | © 2022. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/ The full-text file will be made open to the public on 5 July 2023 in accordance with publisher's 'Terms and Conditions for Self-Archiving' This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/276744 |
DOI(出版社版): | 10.1016/j.bbrc.2022.04.103 |
PubMed ID: | 35504090 |
出現コレクション: | 学術雑誌掲載論文等 |
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