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acs.bioconjchem.2c00307.pdf | 2.32 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Hirose, Hisaaki | en |
dc.contributor.author | Hirai, Yusuke | en |
dc.contributor.author | Sasaki, Michihito | en |
dc.contributor.author | Sawa, Hirofumi | en |
dc.contributor.author | Futaki, Shiroh | en |
dc.contributor.alternative | 廣瀨, 久昭 | ja |
dc.contributor.alternative | 平井, 勇祐 | ja |
dc.contributor.alternative | 二木, 史朗 | ja |
dc.date.accessioned | 2022-10-20T08:23:46Z | - |
dc.date.available | 2022-10-20T08:23:46Z | - |
dc.date.issued | 2022-10-19 | - |
dc.identifier.uri | http://hdl.handle.net/2433/276814 | - |
dc.description.abstract | In precision medicine, extracellular vesicles (EVs) are promising intracellular drug delivery vehicles. The development of a quantitative analysis approach will provide valuable information from the perspective of cell biology and system design for drug delivery. Previous studies have reported quantitative methods to analyze the relative uptake or fusion of EVs to recipient cells. However, relatively few methods have enabled the simultaneous evaluation of the “number” of EVs taken up by recipient cells and those that fuse with cellular membranes. In this study, we report a simple quantitative method based on the NanoBiT system to quantify the uptake and fusion of small and large EVs (sEVs and lEVs, respectively). We assessed the abundance of these two subtypes of EVs and determined that lEVs may be more effective vehicles for transporting cargo to recipient cells. The results also indicated that both sEVs and lEVs have very low fusogenic activity, which can be improved in the presence of a fusogenic protein. | en |
dc.language.iso | eng | - |
dc.publisher | American Chemical Society (ACS) | en |
dc.rights | This document is the Accepted Manuscript version of a Published Work that appeared in final form in Bioconjugate Chemistry, Copyright © 2022 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acs.bioconjchem.2c00307. | en |
dc.rights | The full-text file will be made open to the public on October 4, 2023 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. | en |
dc.rights | This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | en |
dc.subject | Assays | en |
dc.subject | Cell uptake | en |
dc.subject | Membranes | en |
dc.subject | Peptides and proteins | en |
dc.subject | Vesicles | en |
dc.title | Quantitative Analysis of Extracellular Vesicle Uptake and Fusion with Recipient Cells | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Bioconjugate Chemistry | en |
dc.identifier.volume | 33 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 1852 | - |
dc.identifier.epage | 1859 | - |
dc.relation.doi | 10.1021/acs.bioconjchem.2c00307 | - |
dc.textversion | author | - |
dc.identifier.pmid | 36194183 | - |
dcterms.accessRights | open access | - |
datacite.date.available | 2023-10-04 | - |
datacite.awardNumber | 20K22705 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K22705/ | - |
dc.identifier.pissn | 1043-1802 | - |
dc.identifier.eissn | 1520-4812 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | エクソソームと受容細胞との膜融合のリアルタイムイメージング | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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