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ファイル | 記述 | サイズ | フォーマット | |
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eLife.69031.pdf | 7.29 MB | Adobe PDF | 見る/開く |
タイトル: | A mechanism with severing near barbed ends and annealing explains structure and dynamics of dendritic actin networks |
著者: | Holz, Danielle Hall, Aaron R Usukura, Eiji https://orcid.org/0000-0003-0546-4253 (unconfirmed) Yamashiro, Sawako https://orcid.org/0000-0002-5980-0470 (unconfirmed) Watanabe, Naoki https://orcid.org/0000-0001-8492-200X (unconfirmed) Vavylonis, Dimitrios |
著者名の別形: | 山城, 佐和子 臼倉, 英治 渡邊, 直樹 |
キーワード: | Research Article Cell Biology Physics of Living Systems actin polymerization cell motility computational modeling lamellipodium Xenopus |
発行日: | 7-Jun-2022 |
出版者: | eLife Sciences Publications, Ltd |
誌名: | eLife |
巻: | 11 |
論文番号: | e69031 |
抄録: | Single molecule imaging has shown that part of actin disassembles within a few seconds after incorporation into the dendritic filament network in lamellipodia, suggestive of frequent destabilization near barbed ends. To investigate the mechanisms behind network remodeling, we created a stochastic model with polymerization, depolymerization, branching, capping, uncapping, severing, oligomer diffusion, annealing, and debranching. We find that filament severing, enhanced near barbed ends, can explain the single molecule actin lifetime distribution, if oligomer fragments reanneal to free ends with rate constants comparable to in vitro measurements. The same mechanism leads to actin networks consistent with measured filament, end, and branch concentrations. These networks undergo structural remodeling, leading to longer filaments away from the leading edge, at the +/- 35𝑜 orientation pattern. Imaging of actin speckle lifetimes at sub-second resolution verifies frequent disassembly of newly-assembled actin. We thus propose a unified mechanism that fits a diverse set of basic lamellipodia phenomenology. |
著作権等: | © 2022, Holz et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. |
URI: | http://hdl.handle.net/2433/276854 |
DOI(出版社版): | 10.7554/eLife.69031 |
PubMed ID: | 35670664 |
出現コレクション: | 学術雑誌掲載論文等 |
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