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タイトル: | Structural basis of lignocellulose deconstruction by the wood-feeding anobiid beetle Nicobium hirtum |
著者: | Krishanti, Ni Putu Ratna Ayu Tobimatsu, Yuki https://orcid.org/0000-0002-7578-7392 (unconfirmed) Miyamoto, Takuji Fujimoto, Izumi Kartika, Titik Umezawa, Toshiaki Hata, Toshimitsu https://orcid.org/0000-0002-9839-6044 (unconfirmed) Yoshimura, Tsuyoshi |
著者名の別形: | 飛松, 裕基 宮本, 託志 藤本, いずみ 梅澤, 俊明 畑, 俊充 吉村, 剛 |
キーワード: | Anobiid Death-watch beetle Lignocellulose Nicobium hirtum Nuclear magnetic resonance Wood-feeding insect Xylophagous insects |
発行日: | Feb-2022 |
出版者: | Springer Nature The Japan Wood Research Society |
誌名: | Journal of Wood Science |
巻: | 68 |
論文番号: | 10 |
抄録: | The details of the lignocellulose deconstruction processes in the digestive systems of wood-feeding insects remain elusive. This study aimed to examine the biochemical conversion of lignocellulose in the digestive system of a wood-feeding anobiid beetle, Nicobium hirtum, one of the most important pests of wooden products in Japan. To this end, N. hirtum larvae were fed with Japanese red pine (softwood) and Japanese beech (hardwood) sapwood diets, as well as an artificial diet containing Shorea wood (hardwood) sapwood sawdust. The structural differences between the original and digested (feces) lignocellulose samples were examined using wet-chemical and two-dimensional (2D) nuclear magnetic resonance (NMR) methods. Cellulose and hemicelluloses, especially mannan in the softwood diet, were preferentially degraded over lignin in the larval digestive system. As a result, lignin was enriched in the digested lignocellulose residues. Lignin compositional analyses based on thioacidolysis and 2D NMR determined that the proportions of oxidized lignin aromatic units were notably increased after digestion. Further, the 2D NMR analyses revealed the accumulation of aldehyde and hydroxypropiovanillone/syringone end-unit structures in lignin, indicating that oxidative and/or reductive modifications of lignin polymers occur in the larval digestive system. Such structural alterations of lignin may facilitate the dissociation of the lignin barrier, thereby liberating polysaccharides for subsequent enzymatic conversion for assimilation and energy. |
著作権等: | © The Author(s) 2022 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
URI: | http://hdl.handle.net/2433/276975 |
DOI(出版社版): | 10.1186/s10086-022-02017-6 |
出現コレクション: | 学術雑誌掲載論文等 |
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