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j.isci.2022.105427.pdf | 3.16 MB | Adobe PDF | 見る/開く |
タイトル: | Exocyst complex component 2 is a potential host factor for SARS-CoV-2 infection |
著者: | Yi, Renxing Hashimoto, Rina Sakamoto, Ayaka Matsumura, Yasufumi https://orcid.org/0000-0001-8595-8944 (unconfirmed) Nagao, Miki https://orcid.org/0000-0002-8886-6145 (unconfirmed) Takahashi, Kazutoshi Takayama, Kazuo https://orcid.org/0000-0002-1132-2457 (unconfirmed) |
著者名の別形: | 易, 人行 橋本, 里菜 坂本, 綾香 松村, 康史 長尾, 美紀 高橋, 和利 高山, 和雄 |
キーワード: | Human iPS cells airway organoids SARS-CoV-2 EXOC2 CRISPRi IFNW1 COVID-19 |
発行日: | 18-Nov-2022 |
出版者: | Elsevier BV |
誌名: | iScience |
巻: | 25 |
号: | 11 |
論文番号: | 105427 |
抄録: | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an epidemic and spread rapidly all over the world. Because the analysis of host factors other than receptors and proteases has not been sufficiently performed, we attempted to identify and characterize host factors essential for SARS-CoV-2 infection using iPS cells and airway organoids (AO). Based on previous CRISPR screening and RNA-seq data, we found that exocyst complex component 2 (EXOC2) is one important host factor for SARS-CoV-2 infection. The intracellular SARS-CoV-2 nucleocapsid (N) expression level was decreased to 3.7 % and the virus copy number in cell culture medium was decreased to 1.6 % by EXOC2 knockdown. Consistently, immunostaining results showed that N protein-positive cells were significantly decreased by EXOC2 knockdown. We also found that EXOC2 knockdown downregulates SARS-CoV-2 infection by regulating IFNW1 expression. In conclusion, controlling the EXOC2 expression level may prevent SARS-CoV-2 infection and deserves further study. |
記述: | iPS細胞やオルガノイド技術を用いた新型コロナウイルス感染におけるEXOC2の機能解析. 京都大学プレスリリース. 2022-10-27. An important host factor in SARS-CoV-2 infection, identified using iPS cell and organoid technology. 京都大学プレスリリース. 2022-10-31. |
著作権等: | © 2022 The Author(s). This is an open access article under the CC BY-NC-ND license. |
URI: | http://hdl.handle.net/2433/277028 |
DOI(出版社版): | 10.1016/j.isci.2022.105427 |
PubMed ID: | 36310645 |
関連リンク: | https://www.cira.kyoto-u.ac.jp/j/pressrelease/news/221027-110000.html https://www.cira.kyoto-u.ac.jp/e/pressrelease/news/221031-000000.html |
出現コレクション: | 学術雑誌掲載論文等 |
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