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acschembio.2c00221.pdf | 1.7 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Ramasamy, Soundhar | en |
dc.contributor.author | Sahayasheela, Vinodh J. | en |
dc.contributor.author | Sharma, Surbhi | en |
dc.contributor.author | Yu, Zutao | en |
dc.contributor.author | Hidaka, Takuya | en |
dc.contributor.author | Cai, Li | en |
dc.contributor.author | Thangavel, Vaijayanthi | en |
dc.contributor.author | Sugiyama, Hiroshi | en |
dc.contributor.author | Pandian, Ganesh N. | en |
dc.contributor.alternative | 余, 祖滔 | ja |
dc.contributor.alternative | 日髙, 拓也 | ja |
dc.contributor.alternative | 杉山, 弘 | ja |
dc.date.accessioned | 2022-11-16T04:44:09Z | - |
dc.date.available | 2022-11-16T04:44:09Z | - |
dc.date.issued | 2022-10-21 | - |
dc.identifier.uri | http://hdl.handle.net/2433/277273 | - |
dc.description.abstract | Nanopore direct RNA sequencing (dRNA-Seq) reads reveal RNA modifications through consistent error profiles specific to a modified nucleobase. However, a null data set is required to identify actual RNA modification-associated errors for distinguishing it from confounding highly intrinsic sequencing errors. Here, we reveal that inosine creates a signature mismatch error in dRNA-Seq reads and obviates the need for a null data set by harnessing the selective reactivity of acrylonitrile for validating the presence of actual inosine modifications. Selective reactivity of acrylonitrile toward inosine altered multiple dRNA-Seq parameters like signal intensity and trace value. We also deduced the stoichiometry of inosine modification through deviation in signal intensity and trace value using this chemical biology approach. Furthermore, we devised Nano ICE-Seq, a protocol to overcome the low coverage issue associated with direct RNA sequencing. Taken together, our chemical probe-based approach may facilitate the knockout-free detection of disease-associated RNA modifications in clinical scenarios. | en |
dc.language.iso | eng | - |
dc.publisher | American Chemical Society (ACS) | en |
dc.rights | This document is the Accepted Manuscript version of a Published Work that appeared in final form in 'ACS Chemical Biology', copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acschembio.2c00221. | en |
dc.rights | The full-text file will be made open to the public on 3 October 2023 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. | en |
dc.rights | This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | en |
dc.subject | Genetics | en |
dc.subject | Mathematical methods | en |
dc.subject | Modification | en |
dc.subject | Reactivity | en |
dc.subject | Rodent models | en |
dc.title | Chemical Probe-Based Nanopore Sequencing to Selectively Assess the RNA Modifications | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | ACS Chemical Biology | en |
dc.identifier.volume | 17 | - |
dc.identifier.issue | 10 | - |
dc.identifier.spage | 2704 | - |
dc.identifier.epage | 2709 | - |
dc.relation.doi | 10.1021/acschembio.2c00221 | - |
dc.textversion | author | - |
dc.identifier.pmid | 36190780 | - |
dcterms.accessRights | open access | - |
datacite.date.available | 2023-10-03 | - |
datacite.awardNumber | 19H03349 | - |
datacite.awardNumber | 22K19291 | - |
datacite.awardNumber | 21H04705 | - |
datacite.awardNumber | 20H05936 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H03349/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K19291/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21H04705/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-20H05936/ | - |
dc.identifier.pissn | 1554-8929 | - |
dc.identifier.eissn | 1554-8937 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 心筋症関連ミトコンドリア・核内遺伝子の協奏的制御を可能にする人工転写因子の開発 | ja |
jpcoar.awardTitle | リボソームにおけるRNAタンパク質相互作用をマッピングするための統合的アプローチ | ja |
jpcoar.awardTitle | 遺伝子ネットワークを制御する分子の開発と細胞への応用 | ja |
jpcoar.awardTitle | ヌクレオソーム動態のモダリティ | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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