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タイトル: | Interactions of the dynein-2 intermediate chain WDR34 with the light chains are required for ciliary retrograde protein trafficking |
著者: | Tsurumi, Yuta Hamada, Yuki Katoh, Yohei ![]() ![]() Nakayama, Kazuhisa ![]() ![]() |
著者名の別形: | 鶴見, 侑大 濱田, 勇輝 加藤, 洋平 中山, 和久 |
発行日: | 1-Mar-2019 |
出版者: | American Society for Cell Biology (ASCB) |
誌名: | Molecular Biology of the Cell |
巻: | 30 |
号: | 5 |
開始ページ: | 658 |
終了ページ: | 670 |
抄録: | The dynein-2 complex drives retrograde ciliary protein trafficking by associating with the intraflagellar transport (IFT) machinery, containing IFT-A and IFT-B complexes. We recently showed that the dynein-2 complex, which comprises 11 subunits, can be divided into three subcomplexes: DYNC2H1–DYNC2LI1, WDR34–DYNLL1/DYNLL2–DYNLRB1/DYNLRB2, and WDR60–TCTEX1D2–DYNLT1/DYNLT3. In this study, we demonstrated that the WDR34 intermediate chain interacts with the two light chains, DYNLL1/DYNLL2 and DYNLRB1/DYNLRB2, via its distinct sites. Phenotypic analyses of WDR34-knockout cells exogenously expressing various WDR34 constructs showed that the interactions of the WDR34 intermediate chain with the light chains are crucial for ciliary retrograde protein trafficking. Furthermore, we found that expression of the WDR34 N-terminal construct encompassing the light chain–binding sites but lacking the WD40 repeat domain inhibits ciliary biogenesis and retrograde trafficking in a dominant-negative manner, probably by sequestering WDR60 or the light chains. Taken together with phenotypic differences of several WDR34-knockout cell lines, these results indicate that incorporation of DYNLL1/DYNLL2 and DYNLRB1/DYNLRB2 into the dynein-2 complex via interactions with the WDR34 intermediate chain is crucial for dynein-2 function in retrograde ciliary protein trafficking. |
著作権等: | © 2019 Tsurumi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License. |
URI: | http://hdl.handle.net/2433/279637 |
DOI(出版社版): | 10.1091/mbc.E18-10-0678 |
PubMed ID: | 30649997 |
出現コレクション: | 学術雑誌掲載論文等 |
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