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Title: Methods for Estimating Effective Diffusivity of Substrate and Kinetic Parameters of Immobilized Enzyme
Authors: HASHIMOTO, Kenji
ADACHI, Shuji
YOKOYAMA, Masaru
IMAKOMA, Hironobu
GELLF, Gerard
IKEDA, Takahiro
Issue Date: 31-Oct-1985
Publisher: Faculty of Engineering, Kyoto University
Journal title: Memoirs of the Faculty of Engineering, Kyoto University
Volume: 47
Issue: 4
Start page: 276
End page: 289
Abstract: Two methods were presented for estimating simultaneously the kinetic parameters in the Michaelis-Menten equation, Kₘ and Vₘₐₓ, and the intraparticle effective diffusivity of substrate, Dₑᴀ, from the results of the transient changes in a batch reactor. The methods were applied to the estimation of the Kₘ and Vₘₐₓ values of α-chymotrypsin immobilized into firebrick particles or acrylamide gel, and the Dₑᴀ values of substrate through the supports. The experimental data of conversions both in the batch and tubular reactors were found to be calculated successfully by using the kinetic and transport parameters estimated by the proposed methods.
URI: http://hdl.handle.net/2433/281307
Appears in Collections:Vol.47 Part 4

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