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dc.contributor.author | Fujiwara, Takahiro K. | en |
dc.contributor.author | Takeuchi, Shinji | en |
dc.contributor.author | Kalay, Ziya | en |
dc.contributor.author | Nagai, Yosuke | en |
dc.contributor.author | Tsunoyama, Taka A. | en |
dc.contributor.author | Kalkbrenner, Thomas | en |
dc.contributor.author | Iwasawa, Kokoro | en |
dc.contributor.author | Ritchie, Ken P. | en |
dc.contributor.author | Suzuki, Kenichi G.N. | en |
dc.contributor.author | Kusumi, Akihiro | en |
dc.contributor.alternative | 藤原, 敬宏 | ja |
dc.contributor.alternative | 竹内, 信司 | ja |
dc.contributor.alternative | 角山, 貴昭 | ja |
dc.contributor.alternative | 岩沢, こころ | ja |
dc.contributor.alternative | 鈴木, 健一 | ja |
dc.contributor.alternative | 楠見, 明弘 | ja |
dc.date.accessioned | 2023-06-12T01:18:31Z | - |
dc.date.available | 2023-06-12T01:18:31Z | - |
dc.date.issued | 2023-08-07 | - |
dc.identifier.uri | http://hdl.handle.net/2433/283283 | - |
dc.description | 細胞膜上の分子がバレエの群舞のように見えてきた: 1蛍光分子の感度で、究極速度で撮像できるカメラを開発. 京都大学プレスリリース. 2023-06-06. | ja |
dc.description.abstract | The spatial resolution of fluorescence microscopy has recently been greatly enhanced. However, improvements in temporal resolution have been limited, despite their importance for examining living cells. Here, we developed an ultrafast camera system that enables the highest time resolutions in single fluorescent-molecule imaging to date, which were photon-limited by fluorophore photophysics: 33 and 100 µs with single-molecule localization precisions of 34 and 20 nm, respectively, for Cy3, the optimal fluorophore we identified. Using theoretical frameworks developed for the analysis of single-molecule trajectories in the plasma membrane (PM), this camera successfully detected fast hop diffusion of membrane molecules in the PM, previously detectable only in the apical PM using less preferable 40-nm gold probes, thus helping to elucidate the principles governing the PM organization and molecular dynamics. Furthermore, as described in the companion paper, this camera allows simultaneous data acquisitions for PALM/dSTORM at as fast as 1 kHz, with 29/19 nm localization precisions in the 640 × 640 pixel view-field. | en |
dc.language.iso | eng | - |
dc.publisher | Rockefeller University Press | en |
dc.rights | © 2023 Fujiwara et al. | en |
dc.rights | This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Biophysics | en |
dc.subject | Membrane and lipid biology | en |
dc.title | Development of ultrafast camera-based single fluorescent-molecule imaging for cell biology | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Journal of Cell Biology | en |
dc.identifier.volume | 222 | - |
dc.identifier.issue | 8 | - |
dc.relation.doi | 10.1083/jcb.202110160 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | e202110160 | - |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University | en |
dc.address | Photron Limited | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University | en |
dc.address | Photron Limited | en |
dc.address | Membrane Cooperativity Unit, Okinawa Institute of Science and Technology Graduate University (OIST) | en |
dc.address | Carl Zeiss Microscopy GmbH | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University | en |
dc.address | Department of Physics and Astronomy, Purdue University | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University; Institute for Glyco-core Research (iGCORE), Gifu University | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University; Membrane Cooperativity Unit, Okinawa Institute of Science and Technology Graduate University (OIST) | en |
dc.identifier.pmid | 37278763 | - |
dc.relation.url | https://www.icems.kyoto-u.ac.jp/news/8175/ | - |
dcterms.accessRights | open access | - |
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dc.identifier.pissn | 0021-9525 | - |
dc.identifier.eissn | 1540-8140 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 超高速・超解像1蛍光分子局在顕微鏡法による、接着斑の動的群島機構の解明 | ja |
jpcoar.awardTitle | 細胞膜の仕切りが担う、分子複合体形成制御素過程の1分子直接観察 | ja |
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jpcoar.awardTitle | 受容体の超過渡的複合体によるシグナル変換とアクチンによる制御:1分子法による解明 | ja |
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jpcoar.awardTitle | 細胞膜骨格の高速超解像弾性マッピング法の開発と細胞運動解析への応用 | ja |
jpcoar.awardTitle | 超速超高感度・偏光解消顕微鏡の開発によるアクチン膜骨格動態と神経膜拡散障壁の解明 | ja |
jpcoar.awardTitle | 細胞内分子の構造・動態・機能相関を調べるためのクロススケール可視化制御技術の開発 | ja |
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出現コレクション: | 学術雑誌掲載論文等 |
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