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dc.contributor.authorFujiwara, Takahiro K.en
dc.contributor.authorTsunoyama, Taka A.en
dc.contributor.authorTakeuchi, Shinjien
dc.contributor.authorKalay, Ziyaen
dc.contributor.authorNagai, Yosukeen
dc.contributor.authorKalkbrenner, Thomasen
dc.contributor.authorNemoto, Yuri L.en
dc.contributor.authorChen, Limin H.en
dc.contributor.authorShibata, Akihiro C.E.en
dc.contributor.authorIwasawa, Kokoroen
dc.contributor.authorRitchie, Ken P.en
dc.contributor.authorSuzuki, Kenichi G.N.en
dc.contributor.authorKusumi, Akihiroen
dc.contributor.alternative藤原, 敬宏ja
dc.contributor.alternative角山, 貴昭ja
dc.contributor.alternative竹内, 信司ja
dc.contributor.alternative根本, 悠宇里ja
dc.contributor.alternative柴田, 明裕ja
dc.contributor.alternative岩沢, こころja
dc.contributor.alternative鈴木, 健一ja
dc.contributor.alternative楠見, 明弘ja
dc.date.accessioned2023-06-12T01:18:37Z-
dc.date.available2023-06-12T01:18:37Z-
dc.date.issued2023-08-07-
dc.identifier.urihttp://hdl.handle.net/2433/283284-
dc.description細胞膜上の分子がバレエの群舞のように見えてきた: 1蛍光分子の感度で、究極速度で撮像できるカメラを開発. 京都大学プレスリリース. 2023-06-06.ja
dc.description.abstractUsing our newly developed ultrafast camera described in the companion paper, we reduced the data acquisition periods required for photoactivation/photoconversion localization microscopy (PALM, using mEos3.2) and direct stochastic reconstruction microscopy (dSTORM, using HMSiR) by a factor of ≈30 compared with standard methods, for much greater view-fields, with localization precisions of 29 and 19 nm, respectively, thus opening up previously inaccessible spatiotemporal scales to cell biology research. Simultaneous two-color PALM-dSTORM and PALM-ultrafast (10 kHz) single fluorescent-molecule imaging-tracking has been realized. They revealed the dynamic nanoorganization of the focal adhesion (FA), leading to the compartmentalized archipelago FA model, consisting of FA-protein islands with broad diversities in size (13–100 nm; mean island diameter ≈30 nm), protein copy numbers, compositions, and stoichiometries, which dot the partitioned fluid membrane (74-nm compartments in the FA vs. 109-nm compartments outside the FA). Integrins are recruited to these islands by hop diffusion. The FA-protein islands form loose ≈320 nm clusters and function as units for recruiting FA proteins.en
dc.language.isoeng-
dc.publisherRockefeller University Pressen
dc.rights© 2023 Fujiwara et al.en
dc.rightsThis article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).en
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/-
dc.subjectAdhesionen
dc.subjectBiophysicsen
dc.titleUltrafast single-molecule imaging reveals focal adhesion nano-architecture and molecular dynamicsen
dc.typejournal article-
dc.type.niitypeJournal Article-
dc.identifier.jtitleJournal of Cell Biologyen
dc.identifier.volume222-
dc.identifier.issue8-
dc.relation.doi10.1083/jcb.202110162-
dc.textversionpublisher-
dc.identifier.artnume202110162-
dc.addressInstitute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto Universityen
dc.addressMembrane Cooperativity Unit, Okinawa Institute of Science and Technology Graduate University (OIST)en
dc.addressPhotron Limiteden
dc.addressInstitute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto Universityen
dc.addressPhotron Limiteden
dc.addressCarl Zeiss Microscopy GmbHen
dc.addressMembrane Cooperativity Unit, Okinawa Institute of Science and Technology Graduate University (OIST)en
dc.addressMembrane Cooperativity Unit, Okinawa Institute of Science and Technology Graduate University (OIST)en
dc.addressInstitute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto Universityen
dc.addressInstitute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto Universityen
dc.addressDepartment of Physics and Astronomy, Purdue Universityen
dc.addressInstitute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University; Institute for Glyco-core Research, Gifu Universityen
dc.addressInstitute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University; Membrane Cooperativity Unit, Okinawa Institute of Science and Technology Graduate University (OIST)en
dc.identifier.pmid37278764-
dc.relation.urlhttps://www.icems.kyoto-u.ac.jp/news/8175/-
dcterms.accessRightsopen access-
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dc.identifier.pissn0021-9525-
dc.identifier.eissn1540-8140-
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.funderName日本学術振興会ja
jpcoar.awardTitle超高速・超解像1蛍光分子局在顕微鏡法による、接着斑の動的群島機構の解明ja
jpcoar.awardTitle細胞膜の仕切りが担う、分子複合体形成制御素過程の1分子直接観察ja
jpcoar.awardTitle糖脂質による受容体活性制御機構の高精度1分子観察による解明ja
jpcoar.awardTitle高精度1分子観察による糖脂質の機能性クラスター形成機構の解明と階層構造の検証ja
jpcoar.awardTitle受容体の超過渡的複合体によるシグナル変換とアクチンによる制御:1分子法による解明ja
jpcoar.awardTitle超速1分子超解像法による、シグナル経路統合を担う液状ナノ共通シグナル基盤の解明ja
jpcoar.awardTitle細胞膜骨格の高速超解像弾性マッピング法の開発と細胞運動解析への応用ja
jpcoar.awardTitle超速超高感度・偏光解消顕微鏡の開発によるアクチン膜骨格動態と神経膜拡散障壁の解明ja
jpcoar.awardTitle細胞内分子の構造・動態・機能相関を調べるためのクロススケール可視化制御技術の開発ja
jpcoar.awardTitleリポクオリティによるシグナル伝達制御機構の高精度1分子観察による解明ja
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