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dc.contributor.author | Fujiwara, Takahiro K. | en |
dc.contributor.author | Tsunoyama, Taka A. | en |
dc.contributor.author | Takeuchi, Shinji | en |
dc.contributor.author | Kalay, Ziya | en |
dc.contributor.author | Nagai, Yosuke | en |
dc.contributor.author | Kalkbrenner, Thomas | en |
dc.contributor.author | Nemoto, Yuri L. | en |
dc.contributor.author | Chen, Limin H. | en |
dc.contributor.author | Shibata, Akihiro C.E. | en |
dc.contributor.author | Iwasawa, Kokoro | en |
dc.contributor.author | Ritchie, Ken P. | en |
dc.contributor.author | Suzuki, Kenichi G.N. | en |
dc.contributor.author | Kusumi, Akihiro | en |
dc.contributor.alternative | 藤原, 敬宏 | ja |
dc.contributor.alternative | 角山, 貴昭 | ja |
dc.contributor.alternative | 竹内, 信司 | ja |
dc.contributor.alternative | 根本, 悠宇里 | ja |
dc.contributor.alternative | 柴田, 明裕 | ja |
dc.contributor.alternative | 岩沢, こころ | ja |
dc.contributor.alternative | 鈴木, 健一 | ja |
dc.contributor.alternative | 楠見, 明弘 | ja |
dc.date.accessioned | 2023-06-12T01:18:37Z | - |
dc.date.available | 2023-06-12T01:18:37Z | - |
dc.date.issued | 2023-08-07 | - |
dc.identifier.uri | http://hdl.handle.net/2433/283284 | - |
dc.description | 細胞膜上の分子がバレエの群舞のように見えてきた: 1蛍光分子の感度で、究極速度で撮像できるカメラを開発. 京都大学プレスリリース. 2023-06-06. | ja |
dc.description.abstract | Using our newly developed ultrafast camera described in the companion paper, we reduced the data acquisition periods required for photoactivation/photoconversion localization microscopy (PALM, using mEos3.2) and direct stochastic reconstruction microscopy (dSTORM, using HMSiR) by a factor of ≈30 compared with standard methods, for much greater view-fields, with localization precisions of 29 and 19 nm, respectively, thus opening up previously inaccessible spatiotemporal scales to cell biology research. Simultaneous two-color PALM-dSTORM and PALM-ultrafast (10 kHz) single fluorescent-molecule imaging-tracking has been realized. They revealed the dynamic nanoorganization of the focal adhesion (FA), leading to the compartmentalized archipelago FA model, consisting of FA-protein islands with broad diversities in size (13–100 nm; mean island diameter ≈30 nm), protein copy numbers, compositions, and stoichiometries, which dot the partitioned fluid membrane (74-nm compartments in the FA vs. 109-nm compartments outside the FA). Integrins are recruited to these islands by hop diffusion. The FA-protein islands form loose ≈320 nm clusters and function as units for recruiting FA proteins. | en |
dc.language.iso | eng | - |
dc.publisher | Rockefeller University Press | en |
dc.rights | © 2023 Fujiwara et al. | en |
dc.rights | This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Adhesion | en |
dc.subject | Biophysics | en |
dc.title | Ultrafast single-molecule imaging reveals focal adhesion nano-architecture and molecular dynamics | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Journal of Cell Biology | en |
dc.identifier.volume | 222 | - |
dc.identifier.issue | 8 | - |
dc.relation.doi | 10.1083/jcb.202110162 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | e202110162 | - |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University | en |
dc.address | Membrane Cooperativity Unit, Okinawa Institute of Science and Technology Graduate University (OIST) | en |
dc.address | Photron Limited | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University | en |
dc.address | Photron Limited | en |
dc.address | Carl Zeiss Microscopy GmbH | en |
dc.address | Membrane Cooperativity Unit, Okinawa Institute of Science and Technology Graduate University (OIST) | en |
dc.address | Membrane Cooperativity Unit, Okinawa Institute of Science and Technology Graduate University (OIST) | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University | en |
dc.address | Department of Physics and Astronomy, Purdue University | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University; Institute for Glyco-core Research, Gifu University | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University; Membrane Cooperativity Unit, Okinawa Institute of Science and Technology Graduate University (OIST) | en |
dc.identifier.pmid | 37278764 | - |
dc.relation.url | https://www.icems.kyoto-u.ac.jp/news/8175/ | - |
dcterms.accessRights | open access | - |
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dc.identifier.pissn | 0021-9525 | - |
dc.identifier.eissn | 1540-8140 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 超高速・超解像1蛍光分子局在顕微鏡法による、接着斑の動的群島機構の解明 | ja |
jpcoar.awardTitle | 細胞膜の仕切りが担う、分子複合体形成制御素過程の1分子直接観察 | ja |
jpcoar.awardTitle | 糖脂質による受容体活性制御機構の高精度1分子観察による解明 | ja |
jpcoar.awardTitle | 高精度1分子観察による糖脂質の機能性クラスター形成機構の解明と階層構造の検証 | ja |
jpcoar.awardTitle | 受容体の超過渡的複合体によるシグナル変換とアクチンによる制御:1分子法による解明 | ja |
jpcoar.awardTitle | 超速1分子超解像法による、シグナル経路統合を担う液状ナノ共通シグナル基盤の解明 | ja |
jpcoar.awardTitle | 細胞膜骨格の高速超解像弾性マッピング法の開発と細胞運動解析への応用 | ja |
jpcoar.awardTitle | 超速超高感度・偏光解消顕微鏡の開発によるアクチン膜骨格動態と神経膜拡散障壁の解明 | ja |
jpcoar.awardTitle | 細胞内分子の構造・動態・機能相関を調べるためのクロススケール可視化制御技術の開発 | ja |
jpcoar.awardTitle | リポクオリティによるシグナル伝達制御機構の高精度1分子観察による解明 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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