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タイトル: | Isolation, Genomic Sequence and Physiological Characterization of Parageobacillus sp. G301, an Isolate Capable of Both Hydrogenogenic and Aerobic Carbon Monoxide Oxidation |
著者: | Imaura, Yoshinari Okamoto, Shunsuke Hino, Taiki Ogami, Yusuke Katayama, Yuka Adachi Tanimura, Ayumi ![]() ![]() Inoue, Masao Kamikawa, Ryoma ![]() ![]() Yoshida, Takashi ![]() ![]() ![]() Sako, Yoshihiko |
著者名の別形: | 今浦, 由就 岡元, 俊輔 日野, 太貴 大神, 優祐 片山, 夕花 谷村, あゆみ 井上, 真男 神川, 龍馬 吉田, 天士 左子, 芳彦 |
キーワード: | carbon monoxide dehydrogenase carbon monoxide metabolism Parageobacillus |
発行日: | 28-Jun-2023 |
出版者: | American Society for Microbiology |
誌名: | Applied and Environmental Microbiology |
巻: | 89 |
号: | 6 |
論文番号: | e00185-23 |
抄録: | Prokaryotes that can oxidize carbon monoxide (CO oxidizers) can use this gas as a source of carbon or energy. They oxidize carbon monoxide with carbon monoxide dehydrogenases (CODHs): these are divided into nickel-containing CODH (Ni-CODH), which are sensitive to O₂, and molybdenum-containing CODH (Mo-CODH), which can function aerobically. The oxygen conditions required for CO oxidizers to oxidize CO may be limited, as those which have been isolated and characterized so far contain either Ni- or Mo-CODH. Here, we report a novel CO oxidizer, Parageobacillus sp. G301, which is capable of CO oxidation using both types of CODH based on genomic and physiological characterization. This thermophilic, facultatively anaerobic Bacillota bacterium was isolated from the sediments of a freshwater lake. Genomic analyses revealed that strain G301 possessed both Ni-CODH and Mo-CODH. Genome-based reconstruction of its respiratory machinery and physiological investigations indicated that CO oxidation by Ni-CODH was coupled with H₂ production (proton reduction), whereas CO oxidation by Mo-CODH was coupled with O₂ reduction under aerobic conditions and nitrate reduction under anaerobic conditions. G301 would thus be able to thrive via CO oxidation under a wide range of conditions, from aerobic environments to anaerobic environments, even with no terminal electron acceptors other than protons. Comparative genome analyses revealed no significant differences in genome structures and encoded cellular functions, except for CO oxidation between CO oxidizers and non-CO oxidizers in the genus Parageobacillus; CO oxidation genes are retained exclusively for CO metabolism and related respiration. |
著作権等: | © 2023 American Society for Microbiology. All Rights Reserved. The full-text file will be made open to the public on 23 November 2023 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. |
URI: | http://hdl.handle.net/2433/285068 |
DOI(出版社版): | 10.1128/aem.00185-23 |
PubMed ID: | 37219438 |
出現コレクション: | 学術雑誌掲載論文等 |
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