ダウンロード数: 61
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
s41467-023-40934-2.pdf | 6.63 MB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Zhang, Panpan | en |
dc.contributor.author | Maruoka, Masahiro | en |
dc.contributor.author | Suzuki, Ryo | en |
dc.contributor.author | Katani, Hikaru | en |
dc.contributor.author | Dou, Yu | en |
dc.contributor.author | Packwood, Daniel M. | en |
dc.contributor.author | Kosako, Hidetaka | en |
dc.contributor.author | Tanaka, Motomu | en |
dc.contributor.author | Suzuki, Jun | en |
dc.contributor.alternative | チョウ, パンパン | ja |
dc.contributor.alternative | 圓岡, 真宏 | ja |
dc.contributor.alternative | 鈴木, 量 | ja |
dc.contributor.alternative | 蚊⾕, 光 | ja |
dc.contributor.alternative | 童, 友 | ja |
dc.contributor.alternative | パックウッド, ダニエル | ja |
dc.contributor.alternative | ⼩迫, 英尊 | ja |
dc.contributor.alternative | 田中, 求 | ja |
dc.contributor.alternative | 鈴木, 淳 | ja |
dc.date.accessioned | 2023-09-14T02:55:32Z | - |
dc.date.available | 2023-09-14T02:55:32Z | - |
dc.date.issued | 2023-09-11 | - |
dc.identifier.uri | http://hdl.handle.net/2433/285088 | - |
dc.description | 細胞外カルシウムが不要細胞除去に重要! 細胞膜貫通領域どうしを近づけるのりの働きの発⾒. 京都大学プレスリリース. 2023-09-13. | ja |
dc.description | Calcium acts as missing link to dead cell clean-up. 京都大学プレスリリース. 2023-09-13. | en |
dc.description.abstract | The “eat me” signal, phosphatidylserine is exposed on the surface of dying cells by phospholipid scrambling. Previously, we showed that the Xkr family protein Xkr4 is activated by caspase-mediated cleavage and binding of the XRCC4 fragment. Here, we show that extracellular calcium is an additional factor needed to activate Xkr4. The constitutively active mutant of Xkr4 is found to induce phospholipid scrambling in an extracellular, but not intracellular, calcium-dependent manner. Importantly, other Xkr family members also require extracellular calcium for activation. Alanine scanning shows that D123 and D127 of TM1 and E310 of TM3 coordinate calcium binding. Moreover, lysine scanning demonstrates that the E310K mutation-mediated salt bridge between TM1 and TM3 bypasses the requirement of calcium. Cysteine scanning proves that disulfide bond formation between TM1 and TM3 also activates phospholipid scrambling without calcium. Collectively, this study shows that extracellular calcium functions as a molecular glue for TM1 and TM3 of Xkr proteins for activation, thus demonstrating a regulatory mechanism for multi-transmembrane region-containing proteins. | en |
dc.language.iso | eng | - |
dc.publisher | Springer Nature | en |
dc.rights | © The Author(s) 2023 | en |
dc.rights | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Apoptosis | en |
dc.subject | Lipids | en |
dc.subject | Molecular biophysics | en |
dc.title | Extracellular calcium functions as a molecular glue for transmembrane helices to activate the scramblase Xkr4 | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Nature Communications | en |
dc.identifier.volume | 14 | - |
dc.relation.doi | 10.1038/s41467-023-40934-2 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 5592 | - |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University; Graduate School of Biostudies, Kyoto University | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University; Center for Integrated Biosystems, Institute for Biomedical Sciences, Academia Sinica | en |
dc.address | Center for Integrative Medicine and Physics (CiMPhy), Institute for Advanced Study, Kyoto University | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University; Graduate School of Biostudies, Kyoto University | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University | en |
dc.address | Fujii Memorial Institute of Medical Sciences, Institute of Advanced Medical Sciences, Tokushima University | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University; Center for Integrative Medicine and Physics (CiMPhy), Institute for Advanced Study, Kyoto University; Physical Chemistry of Biosystems, Institute of Physical Chemistry, Heidelberg University | en |
dc.address | Institute for Integrated Cell-Material Sciences (WPI-iCeMS), Kyoto University; Graduate School of Biostudies, Kyoto University; Center for Integrated Biosystems, Institute for Biomedical Sciences, Academia Sinica; CREST, Japan Science and Technology Agency | en |
dc.identifier.pmid | 37696806 | - |
dc.relation.url | https://www.icems.kyoto-u.ac.jp/news/8906/ | - |
dc.relation.url | https://www.icems.kyoto-u.ac.jp/en/news/8907/ | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 16H06456 | - |
datacite.awardNumber | 20K06486 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-16H06456/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20K06486/ | - |
dc.identifier.eissn | 2041-1723 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 神経突起コンパートメント化によるスクラップ&ビルド | ja |
jpcoar.awardTitle | 細胞膜リン脂質スクランブラーゼXkr4の活性制御機構の解明 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
このアイテムは次のライセンスが設定されています: クリエイティブ・コモンズ・ライセンス