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Title: Auxin signaling is essential for organogenesis but not for cell survival in the liverwort Marchantia polymorpha
Authors: Suzuki, Hidemasa
Kato, Hirotaka
Iwano, Megumi
Nishihama, Ryuichi
Kohchi, Takayuki
Author's alias: 鈴木, 秀政
加藤, 大貴
岩野, 惠
西浜, 竜一
河内, 孝之
Issue Date: Mar-2023
Publisher: Oxford University Press (OUP)
Journal title: The Plant Cell
Volume: 35
Issue: 3
Start page: 1058
End page: 1075
Abstract: Auxin plays pleiotropic roles in plant development via gene regulation upon its perception by the receptors TRANSPORT INHIBITOR RESPONSE 1/AUXIN SIGNALING F-BOX (TIR1/AFBs). This auxin-regulated transcriptional control mechanism originated in the common ancestor of land plants. Although the complete loss of TIR1/AFBs causes embryonic lethality in Arabidopsis thaliana, it is unclear whether the requirement for TIR1-mediated auxin perception in cell viability can be generalized. The model liverwort Marchantia polymorpha has a minimal auxin signaling system with only a single TIR1/AFB, MpTIR1. Here we show by genetic, biochemical, and transcriptomic analyses that MpTIR1 functions as an evolutionarily conserved auxin receptor. Null mutants and conditionally knocked-out mutants of MpTIR1 were viable but incapable of forming any organs and grew as cell masses. Principal component analysis performed using transcriptomes at various developmental stages indicated that MpTIR1 is involved in the developmental transition from spores to organized thalli, during which apical notches containing stem cells are established. In Mptir1 cells, stem cell- and differentiation-related genes were up- and downregulated, respectively. Our findings suggest that, in M. polymorpha, auxin signaling is dispensable for cell division but is essential for three-dimensional patterning of the plant body by establishing pluripotent stem cells for organogenesis, a derived trait of land plants.
Description: ゼニゴケを用いて植物ホルモンの役割を証明 --オーキシン信号伝達なくして器官形成なし--. 京都大学プレスリリース. 2023-02-07.
Rights: © The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence, which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited.
DOI(Published Version): 10.1093/plcell/koac367
PubMed ID: 36529527
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