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タイトル: | GH20 and GH84 β-N-acetylglucosaminidases with different linkage specificities underpin mucin O-glycan breakdown capability of Bifidobacterium bifidum |
著者: | Takada, Hiromi Katoh, Toshihiko ![]() ![]() ![]() Sakanaka, Mikiyasu ![]() ![]() ![]() Odamaki, Toshitaka Katayama, Takane ![]() ![]() ![]() |
著者名の別形: | 高田, 紘翠 加藤, 紀彦 阪中, 幹祥 小田巻, 俊孝 片山, 高嶺 |
キーワード: | β-N-acetylglucosaminidase Bifidobacterium bifidum glycoside hydrolase mucin mucin O-glycan mass spectrometry substrate specificity |
発行日: | Jun-2023 |
出版者: | Elsevier BV |
誌名: | Journal of Biological Chemistry |
巻: | 299 |
号: | 6 |
論文番号: | 104781 |
抄録: | Intestinal mucous layers mediate symbiosis and dysbiosis of host–microbe interactions. These interactions are influenced by the mucin O-glycan degrading ability of several gut microbes. The identities and prevalence of many glycoside hydrolases (GHs) involved in microbial mucin O-glycan breakdown have been previously reported; however, the exact mechanisms and extent to which these GHs are dedicated to mucin O-glycan degradation pathways warrant further research. Here, using Bifidobacterium bifidum as a model mucinolytic bacterium, we revealed that two β-N-acetylglucosaminidases belonging to the GH20 (BbhI) and GH84 (BbhIV) families play important roles in mucin O-glycan degradation. Using substrate specificity analysis of natural oligosaccharides and O-glycomic analysis of porcine gastric mucin (PGM) incubated with purified enzymes or B. bifidum carrying bbhI and/or bbhIV mutations, we showed that BbhI and BbhIV are highly specific for β-(1→3)- and β-(1→6)-GlcNAc linkages of mucin core structures, respectively. Interestingly, we found that efficient hydrolysis of the β-(1→3)-linkage by BbhI of the mucin core 4 structure [GlcNAcβ1-3(GlcNAcβ1-6)GalNAcα-O-Thr] required prior removal of the β-(1→6)-GlcNAc linkage by BbhIV. Consistent with this, inactivation of bbhIV markedly decreased the ability of B. bifidum to release GlcNAc from PGM. When combined with a bbhI mutation, we observed that the growth of the strain on PGM was reduced. Finally, phylogenetic analysis suggests that GH84 members may have gained diversified functions through microbe–microbe and host–microbe horizontal gene transfer events. Taken together, these data strongly suggest the involvement of GH84 family members in host glycan breakdown. |
著作権等: | © 2023 The Authors. Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology. This is an open access article under the CC BY license. |
URI: | http://hdl.handle.net/2433/285180 |
DOI(出版社版): | 10.1016/j.jbc.2023.104781 |
PubMed ID: | 37146969 |
出現コレクション: | 学術雑誌掲載論文等 |
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