ダウンロード数: 78
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
s00535-023-02006-6.pdf | 1.23 MB | Adobe PDF | 見る/開く |
タイトル: | Anti-integrin αvβ6 autoantibodies in patients with primary sclerosing cholangitis |
著者: | Yoshida, Hiroyuki Shiokawa, Masahiro Kuwada, Takeshi Muramoto, Yuya Ota, Sakiko Nishikawa, Yoshihiro https://orcid.org/0000-0002-1113-6937 (unconfirmed) Maeda, Hirona Kakiuchi, Nobuyuki https://orcid.org/0000-0003-4893-5414 (unconfirmed) Okamoto, Kanako Yamazaki, Hajime https://orcid.org/0000-0002-9034-4370 (unconfirmed) Yokode, Masataka Nakamura, Takeharu Matsumoto, Shimpei Hirano, Tomonori Okada, Hirokazu Marui, Saiko Sogabe, Yuko Matsumori, Tomoaki Mima, Atsushi Uza, Norimitsu Eso, Yuji Takai, Atsushi Takahashi, Ken Ueda, Yoshihide Kodama, Yuzo Chiba, Tsutomu Seno, Hiroshi |
著者名の別形: | 吉田, 裕幸 塩川, 雅広 桒田, 威 村本, 雄哉 太田, 彩貴子 西川, 義浩 前田, 紘奈 垣内, 伸之 山崎, 大 横出, 正隆 中村, 武晴 松本, 慎平 平野, 智紀 岡田, 浩和 丸井, 彩子 曽我部, 裕子 松森, 友昭 美馬, 淳志 宇座, 徳光 恵荘, 裕嗣 髙井, 淳 高橋, 健 千葉, 勉 妹尾, 浩 |
キーワード: | Biomarker Inflammatory bowel diseases Autoimmunity Epithelial cell adhesion molecule Fibronectin |
発行日: | Aug-2023 |
出版者: | Springer Nature |
誌名: | Journal of Gastroenterology |
巻: | 58 |
号: | 8 |
開始ページ: | 778 |
終了ページ: | 789 |
抄録: | [Background] Patients with primary sclerosing cholangitis (PSC) possess autoantibodies against biliary epithelial cells. However, the target molecules remain unknown. [Methods] The sera of patients with PSC and controls were subjected to enzyme-linked immunosorbent assays to detect autoantibodies using recombinant integrin proteins. Integrin αvβ6 expression in the bile duct tissues was examined using immunofluorescence. The blocking activity of the autoantibodies was examined using solid-phase binding assays. [Results] Anti-integrin αvβ6 antibodies were detected in 49/55 (89.1%) patients with PSC and 5/150 (3.3%) controls (P < 0.001), with a sensitivity and specificity of 89.1% and 96.7%, respectively, for PSC diagnosis. When focusing on the presence or absence of IBD, the proportion of the positive antibodies in PSC with IBD was 97.2% (35/36) and that in PSC alone was 73.7% (14/19) (P = 0.008). Integrin αvβ6 was expressed in bile duct epithelial cells. Immunoglobulin (Ig)G from 15/33 patients with PSC blocked integrin αvβ6-fibronectin binding through an RGD (Arg–Gly–Asp) tripeptide motif. [Conclusions] Autoantibodies against integrin αvβ6 were detected in most patients with PSC; anti-integrin αvβ6 antibody may serve as a potential diagnostic biomarker for PSC. |
著作権等: | © The Author(s) 2023 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
URI: | http://hdl.handle.net/2433/285184 |
DOI(出版社版): | 10.1007/s00535-023-02006-6 |
PubMed ID: | 37310456 |
出現コレクション: | 学術雑誌掲載論文等 |
このアイテムは次のライセンスが設定されています: クリエイティブ・コモンズ・ライセンス