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タイトル: | Construction of multiple metagenome assembled genomes containing carbon monoxide dehydrogenases from anaerobic carbon monoxide enrichment cultures |
著者: | Nishida, Shiho Omae, Kimiho Inoue, Masao Sako, Yoshihiko Kamikawa, Ryoma Yoshida, Takashi https://orcid.org/0000-0002-5257-0617 (unconfirmed) |
著者名の別形: | 西田, 志穂 大前, 公保 左子, 芳彦 神川, 龍馬 吉田, 天士 |
キーワード: | Hydrogenogenic carbon monoxide utilizer Carbon monoxide utilizer Carbon monoxide dehydrogenase Metagenome assembled genomes |
発行日: | Aug-2023 |
出版者: | Springer Nature |
誌名: | Archives of Microbiology |
巻: | 205 |
号: | 8 |
論文番号: | 292 |
抄録: | Despite its toxicity to many organisms, including most prokaryotes, carbon monoxide (CO) is utilized by some aerobic and anaerobic prokaryotes. Hydrogenogenic CO utilizers employ carbon monoxide dehydrogenase (CODH) and energy-converting hydrogenase (ECH) to oxidize CO and reduce protons to produce H2. Those prokaryotes constitute a rare biosphere and are difficult to detect even with PCR amplification and with metagenomic analyses. In this study, anaerobic CO-enrichment cultures followed by construction of metagenome assembled genomes (MAGs) detected high-quality MAGs from potential hydrogenogenic CO utilizers. Of 32 MAGs constructed, 5 were potential CO utilizer harboring CODH genes. Of the five MAGs, two were classified into the genus Thermolithobacter on the basis of 16S rRNA sequence identity, related to Carboxydocella tharmautotrophica 41, with an average nucleotide identity (ANI) of approximately 72%. Additionally, two were related to Geoglobus acetivorans with ANI values ranging from 75 to 77% to G. acetivorans SBH6, and one MAG was identified as Desulfotomaculum kuznetsovii with an ANI > 96% to D. kuznetsovii DSM 6115. The two Thermolithobacter MAGs identified in this study contained CODH-ECH gene clusters, and were therefore identified as potential hydrogenogenic CO utilizers. However, these MAGs harbored three CODH gene clusters that showed distinct physiological functions in addition to CODH-ECH gene clusters. In total, the five potential CO utilizer MAGs contained sixteen CODH genes. Among those CODHs, four sets did not cluster with any known CODH protein sequences (with an identity of > 90%), and the CODH database was expanded. |
著作権等: | This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at: http://dx.doi.org/10.1007/s00203-023-03635-4 The full-text file will be made open to the public on 20 July 2024 in accordance with publisher's 'Terms and Conditions for Self-Archiving'. This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 |
URI: | http://hdl.handle.net/2433/285329 |
DOI(出版社版): | 10.1007/s00203-023-03635-4 |
PubMed ID: | 37470847 |
出現コレクション: | 学術雑誌掲載論文等 |
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