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j.celrep.2023.113089.pdf | 8.19 MB | Adobe PDF | 見る/開く |
タイトル: | ActuAtor, a Listeria-inspired molecular tool for physical manipulation of intracellular organizations through de novo actin polymerization |
著者: | Nakamura, Hideki Rho, Elmer Lee, Christopher T. Itoh, Kie Deng, Daqi Watanabe, Satoshi Razavi, Shiva Matsubayashi, Hideaki T. Zhu, Cuncheng Jung, Eleanor Rangamani, Padmini Watanabe, Shigeki Inoue, Takanari |
著者名の別形: | 中村, 秀樹 伊藤, 喜重 渡邉, 哲史 松林, 英明 渡辺, 重喜 井上, 尊生 |
キーワード: | molecular actuators actin polymerization physical manipulation form-function interplay stress granules mitochondria |
発行日: | 31-Oct-2023 |
出版者: | Elsevier BV |
誌名: | Cell Reports |
巻: | 42 |
号: | 10 |
論文番号: | 113089 |
抄録: | Form and function are often interdependent throughout biology. Inside cells, mitochondria have particularly attracted attention since both their morphology and functionality are altered under pathophysiological conditions. However, directly assessing their causal relationship has been beyond reach due to the limitations of manipulating mitochondrial morphology in a physiologically relevant manner. By engineering a bacterial actin regulator, ActA, we developed tools termed “ActuAtor” that inducibly trigger actin polymerization at arbitrary subcellular locations. The ActuAtor-mediated actin polymerization drives striking deformation and/or movement of target organelles, including mitochondria, Golgi apparatus, and nucleus. Notably, ActuAtor operation also disperses non-membrane-bound entities such as stress granules. We then implemented ActuAtor in functional assays, uncovering the physically fragmented mitochondria being slightly more susceptible to degradation, while none of the organelle functions tested are morphology dependent. The modular and genetically encoded features of ActuAtor should enable its application in studies of the form-function interplay in various intracellular contexts. |
記述: | 細胞の中のものを「押す」方法を開発 --細胞内構造体の“かたち”と機能の関係を明らかに--. 京都大学プレスリリース. 2023-09-25. |
著作権等: | © 2023 The Authors. This is an open access article under the CC BY-NC-ND license. |
URI: | http://hdl.handle.net/2433/285964 |
DOI(出版社版): | 10.1016/j.celrep.2023.113089 |
PubMed ID: | 37734382 |
関連リンク: | https://www.t.kyoto-u.ac.jp/ja/research/topics/20230921 |
出現コレクション: | 学術雑誌掲載論文等 |
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