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タイトル: | The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary |
著者: | Tsunemine, Satoru Nakagawa, Hiromi Suzuki, Yutaka Murakami, Yota |
著者名の別形: | 常峰, 悟 中川, 浩実 |
キーワード: | Gene Regulation Chromatin and Epigenetics |
発行日: | 28-Oct-2022 |
出版者: | Oxford University Press (OUP) |
誌名: | Nucleic Acids Research |
巻: | 50 |
号: | 19 |
開始ページ: | 10914 |
終了ページ: | 10928 |
抄録: | Centromeres of most eukaryotes consist of two distinct chromatin domains: a kinetochore domain, identified by the histone H3 variant, CENP-A, and a heterochromatic domain. How these two domains are separated is unclear. Here, we show that, in Schizosaccharomyces pombe, mutation of the chromatin remodeler RSC induced CENP-ACnp1 misloading at pericentromeric heterochromatin, resulting in the mis-assembly of kinetochore proteins and a defect in chromosome segregation. We find that RSC functions at the kinetochore boundary to prevent CENP-ACnp1 from spreading into neighbouring heterochromatin, where deacetylated histones provide an ideal environment for the spread of CENP-ACnp1. In addition, we show that RSC decompacts the chromatin structure at this boundary, and propose that this RSC-directed chromatin decompaction prevents mis-propagation of CENP-ACnp1 into pericentromeric heterochromatin. Our study provides an insight into how the distribution of distinct chromatin domains is established and maintained. |
著作権等: | © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/286138 |
DOI(出版社版): | 10.1093/nar/gkac827 |
PubMed ID: | 36200823 |
出現コレクション: | 学術雑誌掲載論文等 |

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