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タイトル: The chromatin remodeler RSC prevents ectopic CENP-A propagation into pericentromeric heterochromatin at the chromatin boundary
著者: Tsunemine, Satoru
Nakagawa, Hiromi
Suzuki, Yutaka
Murakami, Yota
著者名の別形: 常峰, 悟
中川, 浩実
キーワード: Gene Regulation
Chromatin and Epigenetics
発行日: 28-Oct-2022
出版者: Oxford University Press (OUP)
誌名: Nucleic Acids Research
巻: 50
号: 19
開始ページ: 10914
終了ページ: 10928
抄録: Centromeres of most eukaryotes consist of two distinct chromatin domains: a kinetochore domain, identified by the histone H3 variant, CENP-A, and a heterochromatic domain. How these two domains are separated is unclear. Here, we show that, in Schizosaccharomyces pombe, mutation of the chromatin remodeler RSC induced CENP-ACnp1 misloading at pericentromeric heterochromatin, resulting in the mis-assembly of kinetochore proteins and a defect in chromosome segregation. We find that RSC functions at the kinetochore boundary to prevent CENP-ACnp1 from spreading into neighbouring heterochromatin, where deacetylated histones provide an ideal environment for the spread of CENP-ACnp1. In addition, we show that RSC decompacts the chromatin structure at this boundary, and propose that this RSC-directed chromatin decompaction prevents mis-propagation of CENP-ACnp1 into pericentromeric heterochromatin. Our study provides an insight into how the distribution of distinct chromatin domains is established and maintained.
著作権等: © The Author(s) 2022. Published by Oxford University Press on behalf of Nucleic Acids Research.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
URI: http://hdl.handle.net/2433/286138
DOI(出版社版): 10.1093/nar/gkac827
PubMed ID: 36200823
出現コレクション:学術雑誌掲載論文等

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