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タイトル: | Self-assembly of CIP4 drives actin-mediated asymmetric pit-closing in clathrin-mediated endocytosis |
著者: | Yu, Yiming Yoshimura, Shige H. |
著者名の別形: | 吉村, 成弘 |
キーワード: | Endocytosis Fluorescence imaging Super-resolution microscopy |
発行日: | 1-Aug-2023 |
出版者: | Springer Nature |
誌名: | Nature Communications |
巻: | 14 |
論文番号: | 4602 |
抄録: | Clathrin-mediated endocytosis is pivotal to signal transduction pathways between the extracellular environment and the intracellular space. Evidence from live-cell imaging and super-resolution microscopy of mammalian cells suggests an asymmetric distribution of actin fibres near the clathrin-coated pit, which induces asymmetric pit-closing rather than radial constriction. However, detailed molecular mechanisms of this ‘asymmetricity’ remain elusive. Herein, we used high-speed atomic force microscopy to demonstrate that CIP4, a multi-domain protein with a classic F-BAR domain and intrinsically disordered regions, is necessary for asymmetric pit-closing. Strong self-assembly of CIP4 via intrinsically disordered regions, together with stereospecific interactions with the curved membrane and actin-regulating proteins, generates a small actin-rich environment near the pit, which deforms the membrane and closes the pit. Our results provide mechanistic insights into how disordered and structured domain collaboration promotes spatio-temporal actin polymerisation near the plasma membrane. |
著作権等: | © The Author(s) 2023 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
URI: | http://hdl.handle.net/2433/286623 |
DOI(出版社版): | 10.1038/s41467-023-40390-y |
PubMed ID: | 37528083 |
出現コレクション: | 学術雑誌掲載論文等 |
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