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Title: Recent methodological advances towards single-cell proteomics
Authors: Kim, Sooyeon
Kamarulzaman, Latiefa
Taniguchi, Yuichi  kyouindb  KAKEN_id
Author's alias: 金, 水縁
谷口, 雄一
Keywords: single-cell analysis
single-cell omics
single-molecule microscopy
Issue Date: 11-Oct-2023
Publisher: Japan Academy
Journal title: Proceedings of the Japan Academy, Series B
Volume: 99
Issue: 8
Start page: 306
End page: 327
Abstract: Studying the central dogma at the single-cell level has gained increasing attention to reveal hidden cell lineages and functions that cannot be studied using traditional bulk analyses. Nonetheless, most single-cell studies exploiting genomic and transcriptomic levels fail to address information on proteins that are central to many important biological processes. Single-cell proteomics enables understanding of the functional status of individual cells and is particularly crucial when the specimen is composed of heterogeneous entities of cells. With the growing importance of this field, significant methodological advancements have emerged recently. These include miniaturized and automated sample preparation, multi-omics analyses, and combined analyses of multiple techniques such as mass spectrometry and microscopy. Moreover, artificial intelligence and single-molecule detection technologies have advanced throughput and improved sensitivity limitations, respectively, over conventional methods. In this review, we summarize cutting-edge methodologies for single-cell proteomics and relevant emerging technologies that have been reported in the last 5 years, and provide an outlook on this research field.
Rights: © 2023 The Author(s).
Published under the terms of the CC BY-NC license
DOI(Published Version): 10.2183/pjab.99.021
Appears in Collections:Journal Articles

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