ダウンロード数: 25
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
pjab.99.021.pdf | 9.15 MB | Adobe PDF | 見る/開く |
タイトル: | Recent methodological advances towards single-cell proteomics |
著者: | Kim, Sooyeon Kamarulzaman, Latiefa Taniguchi, Yuichi |
著者名の別形: | 金, 水縁 谷口, 雄一 |
キーワード: | single-cell analysis single-cell omics proteomics single-molecule microscopy |
発行日: | 11-Oct-2023 |
出版者: | Japan Academy |
誌名: | Proceedings of the Japan Academy, Series B |
巻: | 99 |
号: | 8 |
開始ページ: | 306 |
終了ページ: | 327 |
抄録: | Studying the central dogma at the single-cell level has gained increasing attention to reveal hidden cell lineages and functions that cannot be studied using traditional bulk analyses. Nonetheless, most single-cell studies exploiting genomic and transcriptomic levels fail to address information on proteins that are central to many important biological processes. Single-cell proteomics enables understanding of the functional status of individual cells and is particularly crucial when the specimen is composed of heterogeneous entities of cells. With the growing importance of this field, significant methodological advancements have emerged recently. These include miniaturized and automated sample preparation, multi-omics analyses, and combined analyses of multiple techniques such as mass spectrometry and microscopy. Moreover, artificial intelligence and single-molecule detection technologies have advanced throughput and improved sensitivity limitations, respectively, over conventional methods. In this review, we summarize cutting-edge methodologies for single-cell proteomics and relevant emerging technologies that have been reported in the last 5 years, and provide an outlook on this research field. |
著作権等: | © 2023 The Author(s). Published under the terms of the CC BY-NC license |
URI: | http://hdl.handle.net/2433/287048 |
DOI(出版社版): | 10.2183/pjab.99.021 |
出現コレクション: | 学術雑誌掲載論文等 |
このアイテムは次のライセンスが設定されています: クリエイティブ・コモンズ・ライセンス