ダウンロード数: 45
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
adfm.202306070.pdf | 4.42 MB | Adobe PDF | 見る/開く |
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Yoshinaga, Naoto | en |
dc.contributor.author | Miyamoto, Takaaki | en |
dc.contributor.author | Odahara, Masaki | en |
dc.contributor.author | Takeda‐Kamiya, Noriko | en |
dc.contributor.author | Toyooka, Kiminori | en |
dc.contributor.author | Nara, Seia | en |
dc.contributor.author | Nishimura, Haruna | en |
dc.contributor.author | Ling, Feng | en |
dc.contributor.author | Su'etsugu, Masayuki | en |
dc.contributor.author | Yoshida, Minoru | en |
dc.contributor.author | Numata, Keiji | en |
dc.contributor.alternative | 吉永, 直人 | ja |
dc.contributor.alternative | 宮本, 昂明 | ja |
dc.contributor.alternative | 小田原, 真樹 | ja |
dc.contributor.alternative | 武田, 紀子 | ja |
dc.contributor.alternative | 豊岡, 公徳 | ja |
dc.contributor.alternative | 奈良, 聖亜 | ja |
dc.contributor.alternative | 西村, はる菜 | ja |
dc.contributor.alternative | 凌, 楓 | ja |
dc.contributor.alternative | 末次, 正幸 | ja |
dc.contributor.alternative | 吉田, 稔 | ja |
dc.contributor.alternative | 沼田, 圭司 | ja |
dc.date.accessioned | 2024-02-20T02:35:11Z | - |
dc.date.available | 2024-02-20T02:35:11Z | - |
dc.date.issued | 2024-02-19 | - |
dc.identifier.uri | http://hdl.handle.net/2433/287049 | - |
dc.description | 新規ミトコンドリア膜貫通ペプチドによる遺伝子送達 --ミトコンドリア内部で効率的な多重遺伝子発現を達成--. 京都大学プレスリリース. 2023-11-02. | ja |
dc.description.abstract | Mitochondria are vital organelles regulating essential cellular functions. Human mitochondrial DNA (mtDNA) consists of 37 genes, 13 of which encode mitochondrial proteins, and the remaining 24 genes encode two ribosomal RNAs and 22 transfer RNAs needed for the translation of the mtDNA-encoded 13 proteins. However, mtDNA often impairs the expression and function of these genes due to various mutations, ultimately causing mitochondrial dysfunction. To recover from this desperate condition, developing the technology to supply all mitochondrial proteins encoded by mtDNA at once is an urgent task, but there is no established strategy for this purpose. In this study, a simple yet effective mitochondrial gene delivery system is proposed comprising an artificial peptide inspired by a transmembrane mitochondrial membrane protein. The designed mitochondria-targeting peptides presented on the carrier surface effectively guide the encapsulated plasmid to the mitochondria, facilitating mitochondrial uptake and gene expression. The developed system successfully delivers exogenous mtDNA to mtDNA-depleted cells and leads to simultaneous multigene expression, ultimately restoring mitochondrial functions, including the mitochondrial respiration rate. The established multiple gene expression system in each mitochondrion is a game-changing technology that can accelerate the development of mitochondrial engineering technologies as well as clinical applications for mitochondrial diseases. | en |
dc.language.iso | eng | - |
dc.publisher | Wiley | en |
dc.rights | © 2023 The Authors. Advanced Functional Materials published by Wiley-VCH GmbH | en |
dc.rights | This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/4.0/ | - |
dc.subject | gene delivery | en |
dc.subject | mitochondria | en |
dc.subject | mitochondria-targeting peptide | en |
dc.subject | polyplex | en |
dc.title | Design of an Artificial Peptide Inspired by Transmembrane Mitochondrial Protein for Escorting Exogenous DNA into the Mitochondria to Restore their Functions by Simultaneous Multiple Gene Expression | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Advanced Functional Materials | en |
dc.identifier.volume | 34 | - |
dc.identifier.issue | 8 | - |
dc.relation.doi | 10.1002/adfm.202306070 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 2306070 | - |
dc.address | Biomacromolecule Research Team, RIKEN Center for Sustainable Resource Science; Institute for Advanced Biosciences, Keio University | en |
dc.address | Biomacromolecule Research Team, RIKEN Center for Sustainable Resource Science | en |
dc.address | Biomacromolecule Research Team, RIKEN Center for Sustainable Resource Science | en |
dc.address | Technology Platform Division, Mass Spectrometry and Microscopy Unit, RIKEN Center for Sustainable Resource Science | en |
dc.address | Technology Platform Division, Mass Spectrometry and Microscopy Unit, RIKEN Center for Sustainable Resource Science | en |
dc.address | Department of Life Science, College of Science, Rikkyo University | en |
dc.address | Chemical Genomics Research Group, RIKEN Center for Sustainable Resource Science | en |
dc.address | Chemical Genomics Research Group, RIKEN Center for Sustainable Resource Science | en |
dc.address | Department of Life Science, College of Science, Rikkyo University | en |
dc.address | Chemical Genomics Research Group, RIKEN Center for Sustainable Resource Science; Department of Biotechnology, Graduate School of Agricultural and Life Sciences, and Collaborative Research Institute for Innovative Microbiology, The University of Tokyo | en |
dc.address | Biomacromolecule Research Team, RIKEN Center for Sustainable Resource Science; Institute for Advanced Biosciences, Keio University; Department of Material Chemistry, Kyoto University | en |
dc.relation.url | https://www.t.kyoto-u.ac.jp/ja/research/topics/202311011301 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 22H04975 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22H04975/ | - |
dc.identifier.pissn | 1616-301X | - |
dc.identifier.eissn | 1616-3028 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | いつ、どこで、どのように、核酸の高次構造は形成し機能するのかを予測する | ja |
出現コレクション: | 学術雑誌掲載論文等 |
このアイテムは次のライセンスが設定されています: クリエイティブ・コモンズ・ライセンス