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j.cub.2023.12.049.pdf | 8.46 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Hirashima, Tsuyoshi | en |
dc.contributor.author | Matsuda, Michiyuki | en |
dc.contributor.alternative | 平島, 剛志 | ja |
dc.contributor.alternative | 松田, 道行 | ja |
dc.date.accessioned | 2024-02-27T02:56:45Z | - |
dc.date.available | 2024-02-27T02:56:45Z | - |
dc.date.issued | 2024-02-26 | - |
dc.identifier.uri | http://hdl.handle.net/2433/287085 | - |
dc.description | 細胞集団の曲率がカギとなる形作りの仕組み --肺が自律的に枝分かれする原理を提唱--. 京都大学プレスリリース. 2024-02-02. | ja |
dc.description.abstract | Intricate branching patterns emerge in internal organs due to the recurrent occurrence of simple deformations in epithelial tissues. During murine lung development, epithelial cells in distal tips of the single tube require fibroblast growth factor (FGF) signals emanating from their surrounding mesenchyme to form repetitive tip bifurcations. However, it remains unknown how the cells employ FGF signaling to convert their behaviors to achieve the recursive branching processes. Here, we show a mechano-chemical regulatory system underlying lung branching morphogenesis, orchestrated by extracellular signal-regulated kinase (ERK) as a downstream driver of FGF signaling. We found that tissue-scale curvature regulated ERK activity in the lung epithelium using two-photon live cell imaging and mechanical perturbations. ERK activation occurs specifically in epithelial tissues exhibiting positive curvature, regardless of whether the change in curvature was attributable to morphogenesis or perturbations. Moreover, ERK activation accelerates actin polymerization preferentially at the apical side of cells, mechanically contributing to the extension of the apical membrane, culminating in a reduction of epithelial tissue curvature. These results indicate the existence of a negative feedback loop between tissue curvature and ERK activity that transcends spatial scales. Our mathematical model confirms that this regulatory mechanism is sufficient to generate the recursive branching processes. Taken together, we propose that ERK orchestrates a curvature feedback loop pivotal to the self-organized patterning of tissues. | en |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.rights | © 2023 The Author(s). Published by Elsevier Inc. | en |
dc.rights | This is an open access article under the CC BY license. | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | curvature | en |
dc.subject | ERK | en |
dc.subject | FRET biosensor | en |
dc.subject | mechano-chemical feedback | en |
dc.subject | live-cell imaging | en |
dc.subject | lung development | en |
dc.subject | mathematical model | en |
dc.subject | mechanobiology | en |
dc.subject | self-organization | en |
dc.subject | tissue morphogenesis | en |
dc.title | ERK-mediated curvature feedback regulates branching morphogenesis in lung epithelial tissue | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Current Biology | en |
dc.identifier.volume | 34 | - |
dc.identifier.issue | 4 | - |
dc.identifier.spage | 683 | - |
dc.identifier.epage | 696 | - |
dc.relation.doi | 10.1016/j.cub.2023.12.049 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | e6 | - |
dc.address | Mechanobiology Institute, National University of Singapore; Department of Physiology, Yong Loo Lin School of Medicine, National University of Singapore; The Hakubi Center, Kyoto University; Graduate School of Biostudies, Kyoto University; Japan Science and Technology Agency, PRESTO | en |
dc.address | Graduate School of Biostudies, Kyoto University; Graduate School of Medicine, Kyoto University; Institute for Integrated Cell-Material Sciences, Kyoto University | en |
dc.identifier.pmid | 38228149 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2024-02-02 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 19H00993 | - |
datacite.awardNumber | 21H05290 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H00993/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PLANNED-21H05290/ | - |
dc.identifier.pissn | 0960-9822 | - |
dc.identifier.eissn | 1879-0445 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 細胞増殖因子情報伝達系の活性波による細胞集団移動制御機構 | ja |
jpcoar.awardTitle | 多細胞自律性を支える細胞競合機構の数理・物理学的解明 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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