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タイトル: | Encyclopedia of Family A DNA Polymerases Localized in Organelles: Evolutionary Contribution of Bacteria Including the Proto-Mitochondrion |
著者: | Harada, Ryo Hirakawa, Yoshihisa Yabuki, Akinori Kim, Eunsoo Yazaki, Euki Kamikawa, Ryoma ![]() ![]() Nakano, Kentaro Eliáš, Marek Inagaki, Yuji |
著者名の別形: | 原田, 亮 平川, 泰久 矢吹, 彬憲 矢﨑, 裕規 神川, 龍馬 中野, 賢太郎 稲垣, 祐司 |
キーワード: | DNA polymerase endosymbiosis lateral gene transfer last eukaryotic common ancestor mitochondria plastids |
発行日: | Feb-2024 |
出版者: | Oxford University Press (OUP) Society for Molecular Biology and Evolution |
誌名: | Molecular Biology and Evolution |
巻: | 41 |
号: | 2 |
論文番号: | msae014 |
抄録: | DNA polymerases synthesize DNA from deoxyribonucleotides in a semiconservative manner and serve as the core of DNA replication and repair machinery. In eukaryotic cells, there are 2 genome-containing organelles, mitochondria, and plastids, which were derived from an alphaproteobacterium and a cyanobacterium, respectively. Except for rare cases of genome-lacking mitochondria and plastids, both organelles must be served by nucleus-encoded DNA polymerases that localize and work in them to maintain their genomes. The evolution of organellar DNA polymerases has yet to be fully understood because of 2 unsettled issues. First, the diversity of organellar DNA polymerases has not been elucidated in the full spectrum of eukaryotes. Second, it is unclear when the DNA polymerases that were used originally in the endosymbiotic bacteria giving rise to mitochondria and plastids were discarded, as the organellar DNA polymerases known to date show no phylogenetic affinity to those of the extant alphaproteobacteria or cyanobacteria. In this study, we identified from diverse eukaryotes 134 family A DNA polymerase sequences, which were classified into 10 novel types, and explored their evolutionary origins. The subcellular localizations of selected DNA polymerases were further examined experimentally. The results presented here suggest that the diversity of organellar DNA polymerases has been shaped by multiple transfers of the PolI gene from phylogenetically broad bacteria, and their occurrence in eukaryotes was additionally impacted by secondary plastid endosymbioses. Finally, we propose that the last eukaryotic common ancestor may have possessed 2 mitochondrial DNA polymerases, POP, and a candidate of the direct descendant of the proto-mitochondrial DNA polymerase I, rdxPolA, identified in this study. |
記述: | 原始的ミトコンドリアDNA複製酵素の発見. 京都大学プレスリリース. 2024-02-22. |
著作権等: | © The Author(s) 2024. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. |
URI: | http://hdl.handle.net/2433/287088 |
DOI(出版社版): | 10.1093/molbev/msae014 |
PubMed ID: | 38271287 |
関連リンク: | https://www.kyoto-u.ac.jp/ja/research-news/2024-02-22-1 |
出現コレクション: | 学術雑誌掲載論文等 |
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