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j.csbj.2023.10.036.pdf | 10.78 MB | Adobe PDF | 見る/開く |
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dc.contributor.author | Takizawa, Naoki | en |
dc.contributor.author | Kawaguchi, Risa Karakida | en |
dc.contributor.alternative | 滝沢, 直己 | ja |
dc.contributor.alternative | 河口, 理紗 | ja |
dc.date.accessioned | 2024-02-28T02:50:30Z | - |
dc.date.available | 2024-02-28T02:50:30Z | - |
dc.date.issued | 2023 | - |
dc.identifier.uri | http://hdl.handle.net/2433/287105 | - |
dc.description.abstract | The influenza A virus genome is segmented into eight viral RNAs (vRNA). Secondary structures of vRNA are known to be involved in the viral proliferation process. Comprehensive vRNA structures in vitro, in virio, and in cellulo have been analyzed. However, the resolution of the structure map can be improved by comparative analysis and statistical modeling. Construction of a more high-resolution and reliable RNA structure map can identify uncharacterized functional structure motifs on vRNA in virion. Here, we establish the global map of the vRNA secondary structure in virion using the combination of dimethyl sulfate (DMS)-seq and selective 2′-hydroxyl acylation analyzed by primer extension (SHAPE)-seq with a robust statistical analysis. Our high-resolution analysis identified a stem-loop structure at nucleotide positions 39 – 60 of segment 6 and further validated the structure at nucleotide positions 87 – 130 of segment 5 that was previously predicted to form a pseudoknot structure in silico. Notably, when the cells were infected with recombinant viruses which possess the mutations to disrupt the structure, the replication and packaging of the viral genome were drastically decreased. Our results provide comprehensive and high-resolution information on the influenza A virus genome structures in virion and evidence that the functional RNA structure motifs on the influenza A virus genome are associated with appropriate replication and packaging of the viral genome. | en |
dc.language.iso | eng | - |
dc.publisher | Elsevier BV | en |
dc.publisher | Research Network of Computational and Structural Biotechnology | en |
dc.rights | © 2023 The Author(s). Published by Elsevier B.V. on behalf of Research Network of Computational and Structural Biotechnology. | en |
dc.rights | . This is an open access article under the CC BY-NC-ND license. | en |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | - |
dc.subject | Influenza virus | en |
dc.subject | SHAPE-seq | en |
dc.subject | DMS-seq | en |
dc.subject | RNA structure | en |
dc.subject | Chemical probing | en |
dc.title | Comprehensive in virio structure probing analysis of the influenza A virus identifies functional RNA structures involved in viral genome replication | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Computational and Structural Biotechnology Journal | en |
dc.identifier.volume | 21 | - |
dc.identifier.spage | 5259 | - |
dc.identifier.epage | 5272 | - |
dc.relation.doi | 10.1016/j.csbj.2023.10.036 | - |
dc.textversion | publisher | - |
dc.identifier.pmid | 37954152 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 25871077 | - |
datacite.awardNumber | 15K21607 | - |
datacite.awardNumber | 19K07598 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25871077/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-15K21607/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19K07598/ | - |
dc.identifier.eissn | 2001-0370 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | インフルエンザウイルスにおける分節化RNAゲノムパッケージング機構の解析 | ja |
jpcoar.awardTitle | インフルエンザウイルス分節化ゲノムの粒子内立体配置決定 | ja |
jpcoar.awardTitle | インフルエンザウイルスゲノムRNP二次構造のウイルス増殖における機能解析 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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