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journal.pbio.3002006.pdf | 5.6 MB | Adobe PDF | 見る/開く |
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DCフィールド | 値 | 言語 |
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dc.contributor.author | Kaizuka, Takeshi | en |
dc.contributor.author | Hirouchi, Taisei | en |
dc.contributor.author | Saneyoshi, Takeo | en |
dc.contributor.author | Shirafuji, Toshihiko | en |
dc.contributor.author | Collins, Mark O. | en |
dc.contributor.author | Grant, Seth G. N. | en |
dc.contributor.author | Hayashi, Yasunori | en |
dc.contributor.author | Takumi, Toru | en |
dc.contributor.alternative | 貝塚, 剛志 | ja |
dc.contributor.alternative | 廣内, 大成 | ja |
dc.contributor.alternative | 實吉, 岳郎 | ja |
dc.contributor.alternative | 白藤, 俊彦 | ja |
dc.contributor.alternative | 林, 康紀 | ja |
dc.contributor.alternative | 内匠, 透 | ja |
dc.date.accessioned | 2024-03-11T05:10:20Z | - |
dc.date.available | 2024-03-11T05:10:20Z | - |
dc.date.issued | 2024-03 | - |
dc.identifier.uri | http://hdl.handle.net/2433/287296 | - |
dc.description | 神経シナプス後部のタンパク質の集積を司る因子を発見. 京都大学プレスリリース. 2024-03-08. | ja |
dc.description.abstract | Proteome analyses of the postsynaptic density (PSD), a proteinaceous specialization beneath the postsynaptic membrane of excitatory synapses, have identified several thousands of proteins. While proteins with predictable functions have been well studied, functionally uncharacterized proteins are mostly overlooked. In this study, we conducted a comprehensive meta-analysis of 35 PSD proteome datasets, encompassing a total of 5, 869 proteins. Employing a ranking methodology, we identified 97 proteins that remain inadequately characterized. From this selection, we focused our detailed analysis on the highest-ranked protein, FAM81A. FAM81A interacts with PSD proteins, including PSD-95, SynGAP, and NMDA receptors, and promotes liquid–liquid phase separation of those proteins in cultured cells or in vitro. Down-regulation of FAM81A in cultured neurons causes a decrease in the size of PSD-95 puncta and the frequency of neuronal firing. Our findings suggest that FAM81A plays a crucial role in facilitating the interaction and assembly of proteins within the PSD, and its presence is important for maintaining normal synaptic function. Additionally, our methodology underscores the necessity for further characterization of numerous synaptic proteins that still lack comprehensive understanding. | en |
dc.language.iso | eng | - |
dc.publisher | Public Library of Science (PLoS) | en |
dc.rights | © 2024 Kaizuka et al. | en |
dc.rights | This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | - |
dc.subject | Neurons | en |
dc.subject | Hippocampus | en |
dc.subject | Neuronal dendrites | en |
dc.subject | Immunoprecipitation | en |
dc.subject | Proteomes | en |
dc.subject | Condensation | en |
dc.subject | Transfection | en |
dc.subject | Dendritic structure | en |
dc.title | FAM81A is a postsynaptic protein that regulates the condensation of postsynaptic proteins via liquid–liquid phase separation | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | PLOS Biology | en |
dc.identifier.volume | 22 | - |
dc.identifier.issue | 3 | - |
dc.relation.doi | 10.1371/journal.pbio.3002006 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | e3002006 | - |
dc.address | RIKEN Brain Science Institute; Department of Physiology and Cell Biology, Kobe University School of Medicine; Centre for Clinical Brain Sciences, Chancellor’s Building, Edinburgh BioQuarter, University of Edinburgh | en |
dc.address | Department of Pharmacology, Kyoto University Graduate School of Medicine | en |
dc.address | Department of Pharmacology, Kyoto University Graduate School of Medicine | en |
dc.address | Department of Physiology and Cell Biology, Kobe University School of Medicine | en |
dc.address | School of Biosciences, University of Sheffield; biOMICS Facility, Mass Spectrometry Centre, University of Sheffield | en |
dc.address | Centre for Clinical Brain Sciences, Chancellor’s Building, Edinburgh BioQuarter, University of Edinburgh; Simons Initiative for the Developing Brain (SIDB), Centre for Discovery Brain Sciences, University of Edinburgh | en |
dc.address | Department of Pharmacology, Kyoto University Graduate School of Medicine | en |
dc.address | RIKEN Brain Science Institute; Department of Physiology and Cell Biology, Kobe University School of Medicine; RIKEN Center for Biosystems Dynamics Research | en |
dc.identifier.pmid | 38452102 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2024-03-08-1 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 16H06316 | - |
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dc.identifier.eissn | 1545-7885 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 自閉症の生物学的統合研究 | ja |
jpcoar.awardTitle | 精神神経疾患を理解するシナプスのスクラップ&ビルド | ja |
jpcoar.awardTitle | 自閉症ヒト脳オルガノイドの表現解析による病態の基礎的理解 | ja |
jpcoar.awardTitle | 後シナプスタンパク質の発達依存的かつ網羅的データによる臨界期の理解 | ja |
jpcoar.awardTitle | シナプス操作によるPTSDの構成的理解 | ja |
jpcoar.awardTitle | CaMKIIによる液-液相分離を介したシナプス可塑性の調節機構 | ja |
jpcoar.awardTitle | 空間概念の細胞表現「空間文脈細胞」の形成機構と長期空間記憶における役割 | ja |
jpcoar.awardTitle | 樹状突起スパインの刈り込みにおけるオートファジーの役割の解析 | ja |
jpcoar.awardTitle | 発達過程におけるPSD構成因子の変化とシナプス構築の関係 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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