ダウンロード数: 0
このアイテムのファイル:
このアイテムは一定期間後に公開されます。
公開日については,アイテム画面の「著作権等」でご確認ください。
公開日については,アイテム画面の「著作権等」でご確認ください。
完全メタデータレコード
DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | Tsuchida, Arata | en |
dc.contributor.author | Kaneko, Taikopaul | en |
dc.contributor.author | Nishikawa, Kaori | en |
dc.contributor.author | Kawasaki, Mayu | en |
dc.contributor.author | Yokokawa, Ryuji | en |
dc.contributor.author | Shintaku, Hirofumi | en |
dc.contributor.alternative | 土田, 新 | ja |
dc.contributor.alternative | 金子, 泰洸ポール | ja |
dc.contributor.alternative | 横川, 隆司 | ja |
dc.contributor.alternative | 新宅, 博文 | ja |
dc.date.accessioned | 2024-04-23T02:32:17Z | - |
dc.date.available | 2024-04-23T02:32:17Z | - |
dc.date.issued | 2024-04-21 | - |
dc.identifier.uri | http://hdl.handle.net/2433/287554 | - |
dc.description.abstract | We introduce a simple integrated analysis method that links cellular phenotypic behaviour with single-cell RNA sequencing (scRNA-seq) by utilizing a combination of optical indices from cells and hydrogel beads. With our method, the combinations, referred to as joint colour codes, enable the link via matching the optical combinations measured by conventional epi-fluorescence microscopy with the concatenated DNA molecular barcodes created by cell-hydrogel bead pairs and sequenced by next-generation sequencing. We validated our approach by demonstrating an accurate link between the cell image and scRNA-seq with mixed species experiments, longitudinal cell tagging by electroporation and lipofection, and gene expression analysis. Furthermore, we extended our approach to multiplexed chemical transcriptomics, which enabled us to identify distinct phenotypic behaviours in HeLa cells treated with various concentrations of paclitaxel, and determine the corresponding gene regulation associated with the formation of a multipolar spindle. | en |
dc.language.iso | eng | - |
dc.publisher | Royal Society of Chemistry (RSC) | en |
dc.rights | This is an accepted manuscript of the paper which has been published in final form at DOI https://doi.org/10.1039/D3LC00866E | en |
dc.rights | The full-text file will be made open to the public on 20 Mar 2025 in accordance with publisher's 'Terms and Conditions for Self-Archiving' | en |
dc.rights | This is not the published version. Please cite only the published version. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 | en |
dc.title | Opto-combinatorial indexing enables high-content transcriptomics by linking cell images and transcriptomes | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Lab on a Chip | en |
dc.identifier.volume | 24 | - |
dc.identifier.issue | 8 | - |
dc.identifier.spage | 2287 | - |
dc.identifier.epage | 2297 | - |
dc.relation.doi | 10.1039/D3LC00866E | - |
dc.textversion | author | - |
dc.identifier.pmid | 38506394 | - |
dcterms.accessRights | embargoed access | - |
datacite.date.available | 2025-03-20 | - |
datacite.awardNumber | 21K18194 | - |
datacite.awardNumber | 21K14516 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K18194/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K14516/ | - |
dc.identifier.pissn | 1473-0197 | - |
dc.identifier.eissn | 1473-0189 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | ナノ電気穿孔を用いた1細胞ダイナミクス計測法の創成 | ja |
jpcoar.awardTitle | 細胞間相互作用の理解に向けた in situ1細胞タイムラプスRNA-seqの開発 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
このリポジトリに保管されているアイテムはすべて著作権により保護されています。