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DCフィールド | 値 | 言語 |
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dc.contributor.author | Kawakatsu, Yaichi | en |
dc.contributor.author | Okada, Ryo | en |
dc.contributor.author | Hara, Mitsuo | en |
dc.contributor.author | Tsutsui, Hiroki | en |
dc.contributor.author | Yanagisawa, Naoki | en |
dc.contributor.author | Higashiyama, Tetsuya | en |
dc.contributor.author | Arima, Akihide | en |
dc.contributor.author | Baba, Yoshinobu | en |
dc.contributor.author | Kurotani, Ken-ichi | en |
dc.contributor.author | Notaguchi, Michitaka | en |
dc.contributor.alternative | 川勝, 弥一 | ja |
dc.contributor.alternative | 岡田, 龍 | ja |
dc.contributor.alternative | 原, 光生 | ja |
dc.contributor.alternative | 筒井, 大貴 | ja |
dc.contributor.alternative | 柳沢, 直樹 | ja |
dc.contributor.alternative | 東山, 哲也 | ja |
dc.contributor.alternative | 有馬, 彰秀 | ja |
dc.contributor.alternative | 馬場, 嘉信 | ja |
dc.contributor.alternative | 黒谷, 賢一 | ja |
dc.contributor.alternative | 野田口, 理孝 | ja |
dc.date.accessioned | 2024-06-27T06:32:06Z | - |
dc.date.available | 2024-06-27T06:32:06Z | - |
dc.date.issued | 2024-04-03 | - |
dc.identifier.uri | http://hdl.handle.net/2433/287892 | - |
dc.description | 植物の生育状態を野外で早期診断できる装置を開発〜ストレスに応答して生じるmiRNAを葉から検出〜.京都大学プレスリリース. 2024-04-04. | ja |
dc.description.abstract | Plants are exposed to a variety of environmental stress, and starvation of inorganic phosphorus can be a major constraint in crop production. In plants, in response to phosphate deficiency in soil, miR399, a type of microRNA (miRNA), is up-regulated. By detecting miR399, the early diagnosis of phosphorus deficiency stress in plants can be accomplished. However, general miRNA detection methods require complicated experimental manipulations. Therefore, simple and rapid miRNA detection methods are required for early plant nutritional diagnosis. For the simple detection of miR399, microfluidic technology is suitable for point-of-care applications because of its ability to detect target molecules in small amounts in a short time and with simple manipulation. In this study, we developed a microfluidic device to detect miRNAs from filtered plant extracts for the easy diagnosis of plant growth conditions. To fabricate the microfluidic device, verification of the amine-terminated glass as the basis of the device and the DNA probe immobilization method on the glass was conducted. In this device, the target miRNAs were detected by fluorescence of sandwich hybridization in a microfluidic channel. For plant stress diagnostics using a microfluidic device, we developed a protocol for miRNA detection by validating the sample preparation buffer, filtering, and signal amplification. Using this system, endogenous sly-miR399 in tomatoes, which is expressed in response to phosphorus deficiency, was detected before the appearance of stress symptoms. This early diagnosis system of plant growth conditions has a potential to improve food production and sustainability through cultivation management. | en |
dc.language.iso | eng | - |
dc.publisher | American Association for the Advancement of Science (AAAS) | en |
dc.rights | Copyright © 2024 Yaichi Kawakatsu et al. | en |
dc.rights | Exclusive licensee Nanjing Agricultural University. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY 4.0). | en |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | - |
dc.title | Microfluidic Device for Simple Diagnosis of Plant Growth Condition by Detecting miRNAs from Filtered Plant Extracts | en |
dc.type | journal article | - |
dc.type.niitype | Journal Article | - |
dc.identifier.jtitle | Plant Phenomics | en |
dc.identifier.volume | 6 | - |
dc.relation.doi | 10.34133/plantphenomics.0162 | - |
dc.textversion | publisher | - |
dc.identifier.artnum | 0162 | - |
dc.address | Bioscience and Biotechnology Center, Nagoya University | en |
dc.address | Graduate School of Bioagricultural Sciences, Nagoya University | en |
dc.address | Department of Molecular and Macromolecular Chemistry, Graduate School of Engineering, Nagoya University | en |
dc.address | Graduate School of Science, Nagoya University | en |
dc.address | Institute of Transformative Bio-Molecules, Nagoya University | en |
dc.address | Department of Biological Sciences, Graduate School of Science, The University of Tokyo | en |
dc.address | Institute of Nano-Life-Systems, Institutes of Innovation for Future Society, Nagoya University | en |
dc.address | Department of Biomolecular Engineering, Graduate School of Engineering, Nagoya University | en |
dc.address | Institute of Quantum Life Science, National Institutes for Quantum Science and Technology (QST) | en |
dc.address | Department of Botany, Graduate School of Science, Kyoto University | en |
dc.identifier.pmid | 38572468 | - |
dc.relation.url | https://www.kyoto-u.ac.jp/ja/research-news/2024-04-04-0 | - |
dcterms.accessRights | open access | - |
datacite.awardNumber | 21H05657 | - |
datacite.awardNumber | 22H04536 | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-21H05657/ | - |
datacite.awardNumber.uri | https://kaken.nii.ac.jp/grant/KAKENHI-PUBLICLY-22H04536/ | - |
dc.identifier.pissn | 2097-0374 | - |
dc.identifier.eissn | 2643-6515 | - |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.funderName | 日本学術振興会 | ja |
jpcoar.awardTitle | 不均一な環境下で全身移行するmRNAに関する研究 | ja |
jpcoar.awardTitle | 自己集合性ナノ水圏の理解とモルフォロジー制御 | ja |
出現コレクション: | 学術雑誌掲載論文等 |
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