このアイテムのアクセス数: 14
このアイテムのファイル:
ファイル | 記述 | サイズ | フォーマット | |
---|---|---|---|---|
edn3.467.pdf | 3.49 MB | Adobe PDF | 見る/開く |
タイトル: | Detection of fish sedimentary DNA in aquatic systems: A review of methodological challenges and future opportunities |
著者: | Huston, Grayson P. Lopez, Mark Louie D. Cheng, Yuanyu King, Leighton Duxbury, Lucinda C. Picard, Maïlys Thomson-Laing, Georgia Myler, Erika Helbing, Caren C. Kinnison, Michael T. Saros, Jasmine E. Gregory-Eaves, Irene Monchamp, Marie-Eve Wood, Susanna A. Armbrecht, Linda Ficetola, Gentile Francesco Kurte, Lenka Von Eggers, Jordan Brahney, Janice Parent, Genevieve Sakata, Masayuki K. Doi, Hideyuki ![]() ![]() ![]() Capo, Eric |
著者名の別形: | 土居, 秀幸 |
キーワード: | environmental DNA fish monitoring lake sediment marine sediment paleolimnology sedimentary DNA |
発行日: | Nov-2023 |
出版者: | Wiley |
誌名: | Environmental DNA |
巻: | 5 |
号: | 6 |
開始ページ: | 1449 |
終了ページ: | 1472 |
抄録: | Environmental DNA studies have proliferated over the last decade, with promising data describing the diversity of organisms inhabiting aquatic and terrestrial ecosystems. The recovery of DNA present in the sediment of aquatic systems (sedDNA) has provided short- and long-term data on a wide range of biological groups (e.g., photosynthetic organisms, zooplankton species) and has advanced our understanding of how environmental changes have affected aquatic communities. However, substantial challenges remain for recovering the genetic material of macro-organisms (e.g., fish) from sediments, preventing complete reconstructions of past aquatic ecosystems, and limiting our understanding of historic, higher trophic level interactions. In this review, we outline the biotic and abiotic factors affecting the production, persistence, and transport of fish DNA from the water column to the sediments, and address questions regarding the preservation of fish DNA in sediment. We identify sources of uncertainties around the recovery of fish sedDNA arising during the sedDNA workflow. This includes methodological issues related to experimental design, DNA extraction procedures, and the selected molecular method (quantitative PCR, digital PCR, metabarcoding, metagenomics). By evaluating previous efforts (published and unpublished works) to recover fish sedDNA signals, we provide suggestions for future research and propose troubleshooting workflows for the effective detection and quantification of fish sedDNA. With further research, the use of sedDNA has the potential to be a powerful tool for inferring fish presence over time and reconstructing their population and community dynamics. |
著作権等: | © 2023 The Authors. Environmental DNA published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
URI: | http://hdl.handle.net/2433/293418 |
DOI(出版社版): | 10.1002/edn3.467 |
関連リンク: | https://onlinelibrary.wiley.com/doi/pdf/10.1002/edn3.467 |
出現コレクション: | 学術雑誌掲載論文等 |

このアイテムは次のライセンスが設定されています: クリエイティブ・コモンズ・ライセンス