このアイテムのアクセス数: 11
このアイテムのファイル:
| ファイル | 記述 | サイズ | フォーマット | |
|---|---|---|---|---|
| s41467-024-53187-4.pdf | 3.15 MB | Adobe PDF | 見る/開く |
| タイトル: | Molecular mechanism of parental H3/H4 recycling at a replication fork |
| 著者: | Nagae, Fritz Murayama, Yasuto Terakawa, Tsuyoshi    https://orcid.org/0000-0002-0151-1123 (unconfirmed) |
| キーワード: | Computational biophysics Molecular conformation |
| 発行日: | 2-Nov-2024 |
| 出版者: | Springer Nature |
| 誌名: | Nature Communications |
| 巻: | 15 |
| 論文番号: | 9485 |
| 抄録: | In chromatin replication, faithful recycling of histones from parental DNA to replicated strands is essential for maintaining epigenetic information across generations. A previous experiment has revealed that disrupting interactions between the N-terminal tail of Mcm2, a subunit in DNA replication machinery, and a histone H3/H4 tetramer perturb the recycling. However, the molecular pathways and the factors that regulate the ratio recycled to each strand and the destination location are yet to be revealed. Here, we performed molecular dynamics simulations of yeast DNA replication machinery, an H3/H4 tetramer, and replicated DNA strands. The simulations demonstrated that histones are recycled via Cdc45-mediated and unmediated pathways without histone chaperones, as our in vitro biochemical assays supported. Also, RPA binding regulated the ratio recycled to each strand, whereas DNA bending by Pol ε modulated the destination location. Together, the simulations provided testable hypotheses, which are vital for elucidating the molecular mechanisms of histone recycling. |
| 著作権等: | © The Author(s) 2024 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. |
| URI: | http://hdl.handle.net/2433/294079 |
| DOI(出版社版): | 10.1038/s41467-024-53187-4 |
| PubMed ID: | 39488545 |
| 出現コレクション: | 学術雑誌掲載論文等 |
このアイテムは次のライセンスが設定されています: クリエイティブ・コモンズ・ライセンス
