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タイトル: | Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation |
著者: | Ito, Tomoko Iida-Tanaka, Naoko Niidome, Takuro Kawano, Takahito Kubo, Koji Yoshikawa, Kenichi Sato, Toshinori Yang, Zhihong Koyama, Yoshiyuki |
著者名の別形: | 吉川, 研一 |
キーワード: | Transfection Hyaluronic acid Transcription HMG |
発行日: | May-2006 |
出版者: | Elsevier |
引用: | T. Ito, N. Iida-Tanaka, T. Niidome, T. Kawano, K. Kubo, K. Yoshikawa, T. Sato, Z. Yang, and Y. Koyama, “Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation”, J. Controlled Release, 112, 382-388 (2006). |
誌名: | Journal of Controlled Release |
巻: | 112 |
号: | 3 |
開始ページ: | 382 |
終了ページ: | 388 |
抄録: | Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasmid/PEI complexes in CHO cells. |
著作権等: | Copyright © 2006 Elsevier B.V. All rights reserved. この論文は出版社版でありません。引用の際には出版社版をご確認ご利用ください。 This is not the published version. Please cite only the published version. |
URI: | http://hdl.handle.net/2433/49171 |
DOI(出版社版): | 10.1016/j.jconrel.2006.03.013 |
PubMed ID: | 16647780 |
出現コレクション: | 学術雑誌掲載論文等 |
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