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DCフィールド | 値 | 言語 |
---|---|---|
dc.contributor.author | 浅野, 仁子 | ja |
dc.contributor.author | 中村, 正彦 | ja |
dc.contributor.author | 浅野, 朗 | ja |
dc.contributor.alternative | Asano, Kimiko | en |
dc.contributor.alternative | Nakamura, Masahiko | en |
dc.contributor.alternative | Asano, Akira | en |
dc.contributor.transcription | アサノ, キミコ | ja-Kana |
dc.contributor.transcription | ナカムラ, マサヒコ | ja-Kana |
dc.contributor.transcription | アサノ, アキラ | ja-Kana |
dc.date.accessioned | 2007-12-18T06:29:08Z | - |
dc.date.available | 2007-12-18T06:29:08Z | - |
dc.date.issued | 1991 | - |
dc.identifier.issn | 02867850 | - |
dc.identifier.uri | http://hdl.handle.net/2433/49357 | - |
dc.description.abstract | Quantitative measurement of mitochondrial DNA (mtDNA) has been very troublesome, because its content in total DNA was very low and quantitative separation of the mtDNA from nuclear DNA (nucDNA) was almost impossible. By using the technique for cloning cells carrying the mtDNA sequences, specific detection of mtDNA became possible in the presence of large excess of nucDNA by DNA-DNA hybridization. For this purpose, we prepared mtDNA probes labeled with a non-radioactive substrate, which made the procedure easier and increased possibility of application. This method revealed that the contents of mtDNA in rat liver strikingly decreased with aging. | en |
dc.language.iso | jpn | - |
dc.publisher | 京都大学医療技術短期大学部 | ja |
dc.subject.ndc | 492 | - |
dc.title | A Simple Method for Measurement of Mitochondrial DNA Content in Total Cell DNA Using Non-Radioactive DNA Probes | en |
dc.type | departmental bulletin paper | - |
dc.type.niitype | Departmental Bulletin Paper | - |
dc.identifier.ncid | AN00060677 | - |
dc.identifier.jtitle | 京都大学医療技術短期大学部紀要 | ja |
dc.identifier.volume | 11 | - |
dc.identifier.spage | 27 | - |
dc.identifier.epage | 37 | - |
dc.textversion | publisher | - |
dc.sortkey | 03 | - |
dcterms.accessRights | open access | - |
dcterms.alternative | <Originals> A Simple Method for Measurement of Mitochondrial DNA Content in Total Cell DNA Using Non-radioactive DNA Probes | en |
dc.identifier.pissn | 0286-7850 | - |
dc.identifier.jtitle-alternative | Annual reports of the College of Medical Technology, Kyoto University | en |
出現コレクション: | 第11号 |

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