A New Technique For the Histochemical Demonstration of Phosphatase in Hard Tissue

Abstract

A new histochemical technique for the demonstration of alkaline phosphatase in hard tissues was described. This method is characterized by the following three points. First, a neutral mixture solution of tetra- and disodium salt of ethylen diamin tetraacetic acid is adopted as a suitable decalcifying solution for histochemical use. Secondly, neutral formol solution is applied as a reducing reagent for silver phosphate which is produced by histochemical procedure instead of other Kossa's method. Last of all metallic silver which yielded a reaction product is replaced in metallic gold by immersing in a gold chloride solution. By this method, the procedure became easier and excellent figures were obtainable.