Determination of the Major Antigenic Components of Mite (Dermatophagoides farinae) and House Dust Allergens; Non cross-reactivity between the major antigenic components of mite and house dust allergens.

Abstract

The antigenic components of mite (Dermatophagoides farinae, Df) and house dust (HD) allergen were investigated by the Western blotting method. The major antigens were examined with respect to the frequency of binding of the specific IgE antibodies from asthmatic sera. The major antigens of Df had Mr of 16, 24, 28, and 90kD, and the major HD antigen had a Mr of 25kD. Besides having apparently different molecular sizes, these components also had no antigenic cross-reactivity. Therefore, our HD allergen was thought to have the major antigen that has distinct antigenicity from mite allergen. However, we could not clarify the source of our HD major antigen.