<原著>モルモット TDI 喘息モデルにみられた発作死の検討

Abstract

The present paper is a case report on death due to an asthmatic attack found in an experimental model of asthma. The model was developed in guinea pigs by intranasal application of toluene diisocyanate (TDI) for both sensitization and provocation. Among 120 animals with TDI-induced asthma, only one (A-1) died of the attack provoked by the application of TDI. Histopathology of the lungs and the nasal mucosa of A-1 was studied on paraffin-embedded and/or Epon 812-embedded tissue sections. The results were compared with our previous data in other test animals which were sacrificed immediatly after the provocations. While there were some changes common in the lungs of all the test animals including A-1, other changes were seen in only A-1. The former included emphysema, bronchoconstriction, and eosinophilic infiltration, which were similar to pathological changes in asthmatic patients. The latter included narrowing of the airway with hyperplasia of goblet cells, indicative of the cause of A-1's death. The following changes were observed in the nasal mucosa of A-1 : destruction and falling of ciliated cells, intraepithelial edema, hyperplasia of goblet cells, and increase of mast cells. In particular, the former two changes drew our attention, because they had never before been observed in our TDI-induced asthma/nasal allergy model.