<原著>呼吸器の感染防御機構に関する研究 : 第 4 篇 マウス気管支洗浄液中液性成分および抗体産生における喫煙の影響に関する研究

Abstract

In this study, changes in lysozyme, IgA and IgG found in bronchial secretions were examined from mice whose bronchi were stimulated by smoking inhalation. Because the bronchial secretions are irregular in density, when a comparison over time for percentage of total protein is measured, lysozyme in bronchial secretion increases, reacting a peak after 20 minutes. Thus lysozyme secretions from the bronchial gland were considered to have increased due to smoking inhalation. IgA found in bronchial secretions were extremely thin, less than 1/80 of the IgA found in mouse serum, so could not be measured. As for IgG, after 40 minutes of inhalation, there was hardly any change, density being 1/160 of that found in mouse serum. In order to examine the influence of smoking on mouse immunity, especially regarding immunoglobulin production, as an antigen, sheep red blood cells (SRBC) were used, thus examining their effects on plaque forming cells (PFC) in ddy and C57BL/6 mouse spleens. The fact that smoking worked to suppress or enhance IgM PFC production was here confirmed. Finally, recognition of the fact that smoking suppresses or enhances immunoglobulin production against SRBC at the mouse spleen level clearly indicates that smoking possesses not only local effects, but influences immunological reactions throughout the organism.