Partial Purification and Demonstration of Anti-tumor Activity of High Molecular Weight DNA-binding Protein (HMDBP) in Ascitic Fluid of Mice with Meth-A Ascites Fibrosarcoma

Abstract

A high molecular weight DNA-binding protein (HMDBP), from the ascitic fluid of mice with Meth-A ascites, was partially purified with isoelectric focusing column chromatography. The HMDBP was shown to be acidic in isoelectrophoresis. It did not cross-react with IgA, IgM and IgG. The major HMDBP was determined, by sodium dodecylsulfate gel electrophoresis, to be about 160, 000 daltons. In tumor cell cultures, HMDBP suppressed the growth of tumor cells. Therefore, this high molecular weight DNA-binding β-globulin may play a role in controlling tumor growth.