<Original Report>A NEW ATIGEN IN LEUKEMIA L1210 SUBLINE (RESISTANT TO METHYLGLYOXAL-BIS-GUANYLHYDRAZONE)

Abstract

Using paired label radio-antibody method which γ-globulin from antiserum was labeled with ^<125>I and γ-globulin from normal serum was labeled with ^<131>I, the following conclusions had been gotten : 1). Both L1210 and the resistant subline to methylglyoxal-bis-guanylhydrazone (L1210/CH_3-G) cells are immunogenic in vitro and elicit a demonstrable humoral antibody response in the DBA/2Ha-DD mice. 2). The resistant subline L1210/CH_3-G cells is more immunogenic than L1210 cells in DBA/2Ha-DD mice. 3). The antigenic specificity of L1210 is different from that of L1210/CH_3-G cells, although both cell lines share some common antigens. 4). Therefore, the data support the hypothesis that new antigen(s) have been produced in drug-resistant sublines of leukemia L1210.