モルモットにおけるツベルクリン過敏症の受身伝達因子に関する研究

Abstract

It has been demonstrated that passive transfer of tubreculin hypersensitivity is possible by using a certain fraction of spleen extract obtained from guinea pigs vaccinated and challenged with heat-killed BCG. It is suggested that the "Transfer Factor" is high molecular substance which does not pass through cellophane membrane, and that "Inhibitor" is low molecular substance. The behavior on Sephadex columns suggests that the molecular weight of the Transfer Factor is 15, 000&acd;67, 000. This factor seems to be a protein-like substance, because the activity disappears after trypsin treatment but resists RNAse treatment. The histological finding of biopsy material obtained from recipient animals. This is an evidence for successful transfer of tuberculin hypersensitivity. The Transfer Factor is liberated from spleen cells to blood stream by ^<60>Co irradiation (800γ) on the sensitized and challenged guinea pigs. It is concluded that the Transfer Factor of tuberculin hypersensitivity in guinea pigs is very similar to the Transfer Factor found in alveolar macrophages of rabbits which Okada reported already.