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dc.contributor.authorBABA, Kei'ichija
dc.contributor.authorITO, Kazuyaja
dc.date.accessioned2008-04-24T10:19:08Z-
dc.date.available2008-04-24T10:19:08Z-
dc.date.issued1997-09-30ja
dc.identifier.issn0049-7916ja
dc.identifier.urihttp://hdl.handle.net/2433/53204-
dc.descriptionこの論文は国立情報学研究所の学術雑誌公開支援事業により電子化されました。ja
dc.description.abstractThe CTAB method for RNA isolation in differentiating xylem has improved in the treatment of tissues with methanol containing DTT and the addition of vanadyl ribonucleosides in to the extraction buffer. Vanadyl ribonucleosides were supposed to inhibit the binding of polyphenols to RNA also, not only inhibited RNase activity. The yield of RNA was more than 270 μg/g of tissue in average, and poly(A)^+ RNA was subsequently purified by using an oligo-dT cellulose column with about 0.7% recovery of total RNA.ja
dc.language.isoengja
dc.publisherWood Research Institute, Kyoto Universityja
dc.subjectEucalyptusja
dc.subjectRNAja
dc.subjectxylemja
dc.subject.ndc657ja
dc.title<Original>Improvement on Preparation of RNA from Differentiating Xylem Tissue of Eucalyptus camaldulensis L.ja
dc.type.niitypeDepartmental Bulletin Paperja
dc.identifier.ncidAA00890129ja
dc.identifier.jtitleWood research : bulletin of the Wood Research Institute Kyoto Universityja
dc.identifier.volume84ja
dc.identifier.spage7ja
dc.identifier.epage11ja
dc.textversionpublisherja
dc.sortkey06ja
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